Abstract

We demonstrate the capability of the optical coherence tomography (OCT) technique for depth-resolved monitoring and quantifying of glucose diffusion in fibrous tissues (sclera). The depth-resolved and average permeability coefficients of glucose were calculated. We found that the glucose diffusion rate is not uniform throughout the tissue and is increased from approximately 2.39±0.73×106cms at the epithelial side to 8.63±0.27×106cms close to the endothelial side of the sclera. Results demonstrated that the OCT technique is capable of depth-resolved monitoring and quantification of glucose diffusion in sclera with a resolution of approximately 40μm.

© 2006 Optical Society of America

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    [CrossRef]
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    [CrossRef]
  3. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
    [CrossRef] [PubMed]
  4. A. Edwards and M. R. Prausnitz, AIChE J. 44, 214 (1998).
    [CrossRef]
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    [CrossRef]
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    [CrossRef]
  8. W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
    [CrossRef]
  9. A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

2005 (2)

V. V. Tuchin, J. Phys. D 38, 2497 (2005).
[CrossRef]

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

2003 (1)

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

2001 (1)

1998 (2)

M. R. Prausnitz and J. S. Noonan, J. Pharm. Sci. 87, 1479 (1998).
[CrossRef]

A. Edwards and M. R. Prausnitz, AIChE J. 44, 214 (1998).
[CrossRef]

1991 (2)

Y. Komai and T. Ushiki, Invest. Ophthalmol. Visual Sci. 32, 2244 (1991).

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Bashkatov, A. N.

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Choi, B.

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

Edwards, A.

A. Edwards and M. R. Prausnitz, AIChE J. 44, 214 (1998).
[CrossRef]

Esenaliev, R. O.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Genina, E. A.

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Khan, M.

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

Kochubei, V. I.

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

Komai, Y.

Y. Komai and T. Ushiki, Invest. Ophthalmol. Visual Sci. 32, 2244 (1991).

Lakodina, N. A.

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

Larin, K. V.

Larina, I. V.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Motamedi, M.

Nelson, J. S.

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

Noonan, J. S.

M. R. Prausnitz and J. S. Noonan, J. Pharm. Sci. 87, 1479 (1998).
[CrossRef]

Prausnitz, M. R.

M. R. Prausnitz and J. S. Noonan, J. Pharm. Sci. 87, 1479 (1998).
[CrossRef]

A. Edwards and M. R. Prausnitz, AIChE J. 44, 214 (1998).
[CrossRef]

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Sinichkin, Y. P.

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Svaasand, L. O.

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Tuchin, V. V.

V. V. Tuchin, J. Phys. D 38, 2497 (2005).
[CrossRef]

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

V. V. Tuchin, Optical Clearing of Tissues and Blood, Vol. 154 of Press Monograph Series (SPIE, 2005).
[CrossRef]

Ushiki, T.

Y. Komai and T. Ushiki, Invest. Ophthalmol. Visual Sci. 32, 2244 (1991).

Verkruysse, W.

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

AIChE J. (1)

A. Edwards and M. R. Prausnitz, AIChE J. 44, 214 (1998).
[CrossRef]

Biophysics (Engl. Transl.) (1)

A. N. Bashkatov, E. A. Genina, Y. P. Sinichkin, V. I. Kochubei, N. A. Lakodina, and V. V. Tuchin, Biophysics (Engl. Transl.) 48, 292 (2003).

Invest. Ophthalmol. Visual Sci. (1)

Y. Komai and T. Ushiki, Invest. Ophthalmol. Visual Sci. 32, 2244 (1991).

J. Pharm. Sci. (1)

M. R. Prausnitz and J. S. Noonan, J. Pharm. Sci. 87, 1479 (1998).
[CrossRef]

J. Phys. D (1)

V. V. Tuchin, J. Phys. D 38, 2497 (2005).
[CrossRef]

Opt. Lett. (1)

Proc. SPIE (1)

W. Verkruysse, M. Khan, B. Choi, L. O. Svaasand, and J. S. Nelson, in Proc. SPIE 5695, 278 (2005).
[CrossRef]

Science (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Other (1)

V. V. Tuchin, Optical Clearing of Tissues and Blood, Vol. 154 of Press Monograph Series (SPIE, 2005).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

OCT signal slope as a function of time recorded from sclera during the glucose diffusion experiment. The box denotes the times of glucose diffusion in the selected region.

Fig. 2
Fig. 2

OCT signal as a function of time recorded at different depths. The arrows denote the starting point of diffusion at the particular depth in the sclera.

Fig. 3
Fig. 3

Diffusion rates measured at different depths in the sclera. The average standard deviation for the permeability coefficients was ± 0.29 × 10 6 and was calculated from three or four independent experiments.

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