Abstract

We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation.

© 2006 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Simultaneous widefield single molecule orientation and FRET microscopy in cells

S. E. D. Webb, D. J. Rolfe, S. R. Needham, S. K. Roberts, D. T. Clarke, C. I. McLachlan, M. P. Hobson, and M. L. Martin-Fernandez
Opt. Express 16(25) 20258-20265 (2008)

Pulse-shaping based two-photon FRET stoichiometry

Daniel C. Flynn, Amar R. Bhagwat, Meredith H. Brenner, Marcos F. Núñez, Briana E. Mork, Dawen Cai, Joel A. Swanson, and Jennifer P. Ogilvie
Opt. Express 23(3) 3353-3372 (2015)

A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging

Simon P. Poland, Nikola Krstajić, James Monypenny, Simao Coelho, David Tyndall, Richard J. Walker, Viviane Devauges, Justin Richardson, Neale Dutton, Paul Barber, David Day-Uei Li, Klaus Suhling, Tony Ng, Robert K. Henderson, and Simon M. Ameer-Beg
Biomed. Opt. Express 6(2) 277-296 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription