Abstract

An all-mirror dispersion-compensation setup is used to correct for quadratic and cubic phase distortions induced within a custom nonlinear optical microscope. Mouse tail tendon is used to characterize sub-10-fs pulses by interferometric autocorrelation. This is an ideal method for characterizing dispersion from the optical system, immersion medium, and wet biological sample. The generation of very short autocorrelations demonstrates the ability to compensate for phase distortions within the imaging system and efficient second-harmonic upconversion of the ultrashort pulse spectrum within collagen. Compensated autocorrelation traces are presented for biologically relevant objective lenses.

© 2006 Optical Society of America

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M. Muller, J. Squier, R. Wolleschensky, U. Simon, and G. J. Brakenhoff, J. Microsc. 191, 141 (1998).
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W. R. Zipfel, R. M. Williams, and W. W. Webb, Nat. Biotechnol. 21, 1369 (2003).
[CrossRef] [PubMed]

Appl. Opt.

J. Biomed. Opt.

V. Iyer, B. E. Losavio, and P. Saggau, J. Biomed. Opt. 8, 460 (2003).
[CrossRef] [PubMed]

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M. Muller, J. Squier, R. Wolleschensky, U. Simon, and G. J. Brakenhoff, J. Microsc. 191, 141 (1998).
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[CrossRef]

J. Opt. Soc. Am. B

Med. Laser. Appl.

I. Maxwell, S. Chung, and E. Mazur, Med. Laser. Appl. 20, 193 (2005).
[CrossRef]

Nat. Biotechnol.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nat. Biotechnol. 21, 1369 (2003).
[CrossRef] [PubMed]

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Nature

M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, Nature 432, 822 (2004).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Phys. Rev. A

M. Kempe and W. Rudolph, Phys. Rev. A 48, 4721 (1993).
[CrossRef] [PubMed]

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Figures (2)

Fig. 1
Fig. 1

Interferometric autocorrelation of a sub-10-fs laser pulse (inset, from an oscillator) A, after propagation through a 20 × Plan-Apochromat, 0.75 NA objective; B, spectrum measuring 133 nm FWHM centered at 800 nm .

Fig. 2
Fig. 2

Interferometric autocorrelation at the NLOM focal plane using A, 20 × Plan-Apochromat, 0.75 NA ; B, 20 × Achroplan, 0.5 NA ; C, 40 × Achroplan, 0.8 NA ; D, 63 × Achroplan, 0.95 NA ; E, 100 × Achroplan, 1.0 NA ; and F, 63 × C-Apochromat, 1.2 NA . A representative image of mouse tail tendon is shown in the inset of D.

Tables (1)

Tables Icon

Table 1 Calculated Dispersion Parameters, Minimum Pulse Width ( ± 0.4 fs ) , D r , and Total Delay ( ± 2.7 fs ) of the Pulse

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