Abstract

Combining the concept of lateral shear interferometry (LSI) within a digital holography microscope, we demonstrate that it is possible to obtain quantitative optical phase measurement in microscopy by a new single-image-processing procedure. Numerical lateral shear of the reconstructed wavefront in the image plane makes it possible to retrieve the derivative of the wavefront and remove the defocus aberration term introduced by the microscope objective. The method is tested to investigate a silicon structure and a mouse cell line.

© 2006 Optical Society of America

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2005

2004

2003

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

P. Ferraro, G. Coppola, S. DeNicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
[CrossRef] [PubMed]

A. Dakoff, J. Gass, and M. K. Kim, J. Electron. Imaging 12, 643 (2003).
[CrossRef]

2002

2001

L. Xu, X. Peng, J. Miao, and A. K. Asundi, Appl. Opt. 40, 5046 (2001).
[CrossRef]

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

2000

S. De Nicola and P. Ferraro, Opt. Commun. 185, 285 (2000).
[CrossRef]

A. Stadelmaier and J. H. Massig, Opt. Lett. 25, 1630 (2000).
[CrossRef]

1999

1998

1992

Z. J. L. Ramirez, M. F. Castro, and H. W. Kuri, Histochemistry 97, 493 (1992).
[CrossRef]

Akkin, T.

Asundi, A. K.

Badizadegan, K.

Barty, A.

Baxter, G.

Carapezza, E.

Castro, M. F.

Z. J. L. Ramirez, M. F. Castro, and H. W. Kuri, Histochemistry 97, 493 (1992).
[CrossRef]

Cense, B.

Colomb, T.

Coppola, G.

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

P. Ferraro, G. Coppola, S. DeNicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
[CrossRef] [PubMed]

Cuche, E.

Dakoff, A.

A. Dakoff, J. Gass, and M. K. Kim, J. Electron. Imaging 12, 643 (2003).
[CrossRef]

Dasari, R. R.

de Boer, J. F.

De Nicola, S.

S. De Nicola and P. Ferraro, Opt. Commun. 185, 285 (2000).
[CrossRef]

Deflores, L. P.

DeNicola, S.

P. Ferraro, G. Coppola, S. DeNicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
[CrossRef] [PubMed]

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

Depeursinge, C.

Dragomir, N.

Dubois, F.

Emery, Y.

Farrel, P.

Feld, M. S.

Ferraro, P.

P. Ferraro, G. Coppola, S. DeNicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
[CrossRef] [PubMed]

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

S. De Nicola and P. Ferraro, Opt. Commun. 185, 285 (2000).
[CrossRef]

Finizio, A.

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

P. Ferraro, G. Coppola, S. DeNicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
[CrossRef] [PubMed]

Gass, J.

A. Dakoff, J. Gass, and M. K. Kim, J. Electron. Imaging 12, 643 (2003).
[CrossRef]

Grilli, S.

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

Ikeda, T.

Iwai, H.

Javidi, B.

Joo, C.

Kim, M. K.

A. Dakoff, J. Gass, and M. K. Kim, J. Electron. Imaging 12, 643 (2003).
[CrossRef]

Kuri, H. W.

Z. J. L. Ramirez, M. F. Castro, and H. W. Kuri, Histochemistry 97, 493 (1992).
[CrossRef]

Legros, J. C.

Magistretti, P.

Magro, C.

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

Mantravadi, M. V.

M. V. Mantravadi, in Optical Shop Testing, 2nd ed., D.Malacara, ed. (Wiley Interscience, 1992), Chap. 4, pp. 123-172.

Marquet, P.

Massig, J. H.

Miao, J.

Michna, M. L.

Monnom, O.

Moon, I.

Nugent, K. A.

Paganin, D.

Park, B. H.

Pedrini, G.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Peng, X.

Pierattini, G.

P. Ferraro, G. Coppola, S. DeNicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
[CrossRef] [PubMed]

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

Popescu, G.

Ramirez, Z. J. L.

Z. J. L. Ramirez, M. F. Castro, and H. W. Kuri, Histochemistry 97, 493 (1992).
[CrossRef]

Rappaz, B.

Roberts, A.

Schedin, S.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Stadelmaier, A.

Thorn, K.

Tiziani, H. J.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Vaughan, J. C.

Xu, L.

Yamaguchi, I.

Yeom, S.

Yourassowsky, C.

Zhang, T.

Appl. Opt.

Appl. Phys. Lett.

P. Ferraro, S. DeNicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Phys. Lett. 42, 1936 (2003).

Histochemistry

Z. J. L. Ramirez, M. F. Castro, and H. W. Kuri, Histochemistry 97, 493 (1992).
[CrossRef]

J. Electron. Imaging

A. Dakoff, J. Gass, and M. K. Kim, J. Electron. Imaging 12, 643 (2003).
[CrossRef]

J. Mod. Opt.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Opt. Commun.

S. De Nicola and P. Ferraro, Opt. Commun. 185, 285 (2000).
[CrossRef]

Opt. Express

Opt. Lett.

Other

M. V. Mantravadi, in Optical Shop Testing, 2nd ed., D.Malacara, ed. (Wiley Interscience, 1992), Chap. 4, pp. 123-172.

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Figures (4)

Fig. 1
Fig. 1

DH setup; S’s, samples; BSs, beam splitters; MOs, microscope objectives.

Fig. 2
Fig. 2

(a) Digital hologram. Phase shearograms (b) in the reconstructed plane and (c) with tilt removed. (d) QPM photo of the profile of the MEMS by LSI with DH. (e) Wrapped phase map obtained by a double exposure approach[15] and (f) its unwrapped phase map.

Fig. 3
Fig. 3

Shearogram along the (a) x and (b) y directions. (c) QPM photo of a cell and (d) its three-dimensional plot. An arrow indicates a lipid particle detected in the cell line.

Fig. 4
Fig. 4

Shearogram along (a) the x and (b) the y directions; (c) QPM photo of a cell with lipid accumulation and (d) its three-dimensional plot.

Equations (6)

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ϕ ( x , y ) = ϕ O ( x , y ) + i k 2 R ( x 2 + y 2 ) ,
ϕ ( x , y ) x Δ ϕ x s x ,
ϕ ( x , y ) y Δ ϕ y s y .
ϕ ( x , y ) y ϕ O ( x , y ) ϕ O ( x s x , y ) s x + i k s x x R ,
ϕ ( x , y ) y ϕ O ( x , y ) ϕ O ( x s x , y s y ) s y + i k s y y R .
ϕ O ( x + Δ x , y + Δ y ) Φ O ( x , y ) + Δ ϕ O ( x , y ) Δ x + Δ ϕ O ( x , y ) Δ y

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