We report on the applicability of combining surface-enhanced Raman scattering (SERS) with coherent anti-Stokes Raman scattering for high-sensitivity detection of biological molecules. We found that this combination of techniques provides more than 3 orders of signal enhancement compared with SERS and permits monitoring of biological molecules such as deoxyguanosine monophosphate (dGMP) and deoxyadenosine monophosphate at the single-molecule level. This combined technique also improved detection sensitivity for angiotensin peptide. As this is believed to be the first report of detection of dGMP at the single-molecule level, we suggest that this approach can serve as a new tool for biological studies.
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H. Chew, D.-S. Wang, and M. Kerker
J. Opt. Soc. Am. B 1(1) 56-66 (1984)
X. Dou, T. Takama, Y. Yamaguchi, K. Hirai, H. Yamamoto, S. Doi, and Y. Ozaki
Appl. Opt. 37(4) 759-763 (1998)
Daniel J. White and Paul R. Stoddart
Opt. Lett. 30(6) 598-600 (2005)