Abstract

The capability of photothermal (PT) microscopy to image moving, unlabeled cells in real time in vivo is demonstrated in a study of circulating red and white blood cells in blood and lymph microvessels of rat mesentery. Potential applications of this optical tool, called PT flow cytometry, are discussed.

© 2005 Optical Society of America

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References

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  1. W. Rudolph and M. Kempe, J. Mod. Opt. 44, 1617 (1997).
    [CrossRef]
  2. D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
    [CrossRef] [PubMed]
  3. D. Lapotko, T. Romanovskaya, and V. Zharov, J. Biomed. Opt. 7, 425 (2002).
    [CrossRef] [PubMed]
  4. V. P. Zharov, in Laser Analytical Spectrochemistry, V. S. Letokhov, ed. (Hilger, Boston, 1986), p. 229.
  5. V. Zharov and D. Lapotko, Rev. Sci. Instrum. 74, 785 (2003).
    [CrossRef]
  6. V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).
    [CrossRef]
  7. A. L. Givan, Methods Cell Biol. 63, 19 (2001).
    [CrossRef]

2003

V. Zharov and D. Lapotko, Rev. Sci. Instrum. 74, 785 (2003).
[CrossRef]

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).
[CrossRef]

2002

D. Lapotko, T. Romanovskaya, and V. Zharov, J. Biomed. Opt. 7, 425 (2002).
[CrossRef] [PubMed]

2001

A. L. Givan, Methods Cell Biol. 63, 19 (2001).
[CrossRef]

1997

W. Rudolph and M. Kempe, J. Mod. Opt. 44, 1617 (1997).
[CrossRef]

1996

D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
[CrossRef] [PubMed]

Galitovsky, V.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).
[CrossRef]

Givan, A. L.

A. L. Givan, Methods Cell Biol. 63, 19 (2001).
[CrossRef]

Kempe, M.

W. Rudolph and M. Kempe, J. Mod. Opt. 44, 1617 (1997).
[CrossRef]

Kuchinsky, G.

D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
[CrossRef] [PubMed]

Lapotko, D.

V. Zharov and D. Lapotko, Rev. Sci. Instrum. 74, 785 (2003).
[CrossRef]

D. Lapotko, T. Romanovskaya, and V. Zharov, J. Biomed. Opt. 7, 425 (2002).
[CrossRef] [PubMed]

D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
[CrossRef] [PubMed]

Pechkovsky, D.

D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
[CrossRef] [PubMed]

Potapnev, M.

D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
[CrossRef] [PubMed]

Romanovskaya, T.

D. Lapotko, T. Romanovskaya, and V. Zharov, J. Biomed. Opt. 7, 425 (2002).
[CrossRef] [PubMed]

Rudolph, W.

W. Rudolph and M. Kempe, J. Mod. Opt. 44, 1617 (1997).
[CrossRef]

Viegas, M.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).
[CrossRef]

Zharov, V.

V. Zharov and D. Lapotko, Rev. Sci. Instrum. 74, 785 (2003).
[CrossRef]

D. Lapotko, T. Romanovskaya, and V. Zharov, J. Biomed. Opt. 7, 425 (2002).
[CrossRef] [PubMed]

Zharov, V. P.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).
[CrossRef]

V. P. Zharov, in Laser Analytical Spectrochemistry, V. S. Letokhov, ed. (Hilger, Boston, 1986), p. 229.

Appl. Phys. Lett.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).
[CrossRef]

Cytometry

D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, Cytometry 24, 198 (1996).
[CrossRef] [PubMed]

J. Biomed. Opt.

D. Lapotko, T. Romanovskaya, and V. Zharov, J. Biomed. Opt. 7, 425 (2002).
[CrossRef] [PubMed]

J. Mod. Opt.

W. Rudolph and M. Kempe, J. Mod. Opt. 44, 1617 (1997).
[CrossRef]

Methods Cell Biol.

A. L. Givan, Methods Cell Biol. 63, 19 (2001).
[CrossRef]

Rev. Sci. Instrum.

V. Zharov and D. Lapotko, Rev. Sci. Instrum. 74, 785 (2003).
[CrossRef]

Other

V. P. Zharov, in Laser Analytical Spectrochemistry, V. S. Letokhov, ed. (Hilger, Boston, 1986), p. 229.

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Figures (4)

Fig. 1
Fig. 1

Schematic of the integrated laser PT microscope–spectrometer.

Fig. 2
Fig. 2

Bottom, typical image of rat mesentery with blood and lymph microvessels. Top left, RBCs in a capillary. Top right, cells in lymph flow.

Fig. 3
Fig. 3

Optical transmission (left column) and PT (right column) images in vivo of a single, moving lymphocyte (top row) and RBC (bottom row) in lymph flow in rat mesentery (vessel diameter, 105 μ m , velocity, 120 μ m s ). Pump–pulse parameters: wavelength, 525 nm ; pulse width, 8 ns ; pulse energies, 30 and 0.5 μ J (top and bottom, respectively); time delay, 10 ns .

Fig. 4
Fig. 4

Top row, optical (first) and PT (second) images of blood microvessels with a relatively high flow velocity ( > 1 mm s ) . Bottom row: first, PT image of blood vessels without cells; second, PT image of microlymphatics with 20 - nm gold nanoparticles. The dashed circles show the position of the probe beam. Pump laser parameters:  wavelength, 525 nm ; pulse width, 8 ns ; pulse energy, 0.5 μ J (second, top row) and 100 μ J (first and second, bottom row); time delay, 10 ns .

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