Abstract

The thermal disruption of collagen I in rat tail tendon is investigated with second-harmonic generation (SHG) microscopy. We investigate its effects on SHG images and intensity in the temperature range 25°60°C. We find that the SHG signal decreases rapidly starting at 45°C. However, SHG imaging reveals that breakage of collagen fibers is not evident until 57°C and worsens with increasing temperature. At 57°C, structures of both molten and fibrous collagen exist, and the disruption of collagen appears to be complete at 60°C. Our results suggest that, in addition to intensity measurement, SHG imaging is necessary for monitoring details of thermally induced changes in collagen structures in biomedical applications.

© 2005 Optical Society of America

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A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

2003

2002

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef]

2001

T. R. Lyons, P. L. Griffith, F. H. Savoie, and L. D. Field, Arthroscopy 17, 25 (2001).
[CrossRef] [PubMed]

2000

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

1996

R. E. Fitzpatrick, M. P. Goldman, N. M. Satur, and W. D. Tope, Arch. Dermatol. 132, 395 (1996).
[CrossRef] [PubMed]

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W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Berland, K. M.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Campagnola, P. J.

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Chen, Z.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

Da Silva, L. B.

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

Davidorf, J.

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Dong, C. Y.

Eichler, J.

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

Field, L. D.

T. R. Lyons, P. L. Griffith, F. H. Savoie, and L. D. Field, Arthroscopy 17, 25 (2001).
[CrossRef] [PubMed]

Fitzpatrick, R. E.

R. E. Fitzpatrick, M. P. Goldman, N. M. Satur, and W. D. Tope, Arch. Dermatol. 132, 395 (1996).
[CrossRef] [PubMed]

Georgiou, E.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Goldman, M. P.

R. E. Fitzpatrick, M. P. Goldman, N. M. Satur, and W. D. Tope, Arch. Dermatol. 132, 395 (1996).
[CrossRef] [PubMed]

Griffith, P. L.

T. R. Lyons, P. L. Griffith, F. H. Savoie, and L. D. Field, Arthroscopy 17, 25 (2001).
[CrossRef] [PubMed]

Hersh, P. S.

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Hovhannisyan, V.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Jee, S. H.

Jung, W. G.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

Kao, B.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

Kim, B. M.

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

Lin, S. J.

Lo, W.

Loew, L. M.

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Lyons, T. R.

T. R. Lyons, P. L. Griffith, F. H. Savoie, and L. D. Field, Arthroscopy 17, 25 (2001).
[CrossRef] [PubMed]

Maloney, R. K.

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Manche, E. E.

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Masters, B. R.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

McDonald, M. B.

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Politopoulos, K.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Rapti, G. S.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Reiser, K. M.

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

Rubenchik, A. M.

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

Sabry, M.

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Satur, N. M.

R. E. Fitzpatrick, M. P. Goldman, N. M. Satur, and W. D. Tope, Arch. Dermatol. 132, 395 (1996).
[CrossRef] [PubMed]

Savoie, F. H.

T. R. Lyons, P. L. Griffith, F. H. Savoie, and L. D. Field, Arthroscopy 17, 25 (2001).
[CrossRef] [PubMed]

So, P. T. C.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Stuart, J. S.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

Su, J. W.

Sun, Y.

Theodossiou, T.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Tope, W. D.

R. E. Fitzpatrick, M. P. Goldman, N. M. Satur, and W. D. Tope, Arch. Dermatol. 132, 395 (1996).
[CrossRef] [PubMed]

Tromberg, B. J.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef]

Webb, W. W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Yeh, A.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef]

Yeh, A. T.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

Yova, D.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Zoumi, A.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef]

Annu. Rev. Biomed. Eng.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Arch. Dermatol.

R. E. Fitzpatrick, M. P. Goldman, N. M. Satur, and W. D. Tope, Arch. Dermatol. 132, 395 (1996).
[CrossRef] [PubMed]

Arthroscopy

T. R. Lyons, P. L. Griffith, F. H. Savoie, and L. D. Field, Arthroscopy 17, 25 (2001).
[CrossRef] [PubMed]

J. Biomed. Opt.

A. T. Yeh, B. Kao, W. G. Jung, Z. Chen, J. S. Stuart, and B. J. Tromberg, J. Biomed. Opt. 9, 248 (2004).
[CrossRef] [PubMed]

Lasers Med. Sci.

T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, Lasers Med. Sci. 17, 34 (2002).
[CrossRef]

Lasers Surg. Med.

B. M. Kim, J. Eichler, K. M. Reiser, A. M. Rubenchik, and L. B. Da Silva, Lasers Surg. Med. 27, 329 (2000).
[CrossRef]

Nat. Biotechnol.

P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
[CrossRef] [PubMed]

Ophthalmology

M. B. McDonald, P. S. Hersh, E. E. Manche, R. K. Maloney, J. Davidorf, and M. Sabry, and the Conductive Keratoplasty United States Investigators Group, Ophthalmology 109, 1978 (2002).
[CrossRef]

Opt. Express

Proc. Natl. Acad. Sci. USA

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef]

Science

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

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Figures (2)

Fig. 1
Fig. 1

SHG images ( 390 nm ) of rat tail tendon treated for 10 min at different temperatures. Disruption of collagen structures are indicated by arrows. Histological images are shown for comparison.

Fig. 2
Fig. 2

Dependence of the SHG signal ( 390 nm ) of rat tail tendon at different temperatures.

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