The thermal disruption of collagen I in rat tail tendon is investigated with second-harmonic generation (SHG) microscopy. We investigate its effects on SHG images and intensity in the temperature range . We find that the SHG signal decreases rapidly starting at . However, SHG imaging reveals that breakage of collagen fibers is not evident until and worsens with increasing temperature. At , structures of both molten and fibrous collagen exist, and the disruption of collagen appears to be complete at . Our results suggest that, in addition to intensity measurement, SHG imaging is necessary for monitoring details of thermally induced changes in collagen structures in biomedical applications.
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