Abstract
We derive a novel algorithm to recover the in vivo distributions of fluorophores based on an asymptotic lifetime analysis of time-domain fluorescence measurements with turbid tissue. We experimentally demonstrate the advantage offered by this method in localizing fluorophores with distinct lifetimes. This algorithm has wide applicability for diagnostic fluorescence imaging in the presence of several-centimeter-thick biological tissue, since fluorescence lifetime is a sensitive indicator of local tissue environment and interactions at the molecular level.
© 2005 Optical Society of America
Full Article | PDF ArticleMore Like This
Anand T. N. Kumar, Scott B. Raymond, Gregory Boverman, David A. Boas, and Brian J. Bacskai
Opt. Express 14(25) 12255-12270 (2006)
Eric Shives, Yong Xu, and Huabei Jiang
Opt. Express 10(26) 1557-1562 (2002)
M. A. O’Leary, D. A. Boas, X. D. Li, B. Chance, and A. G. Yodh
Opt. Lett. 21(2) 158-160 (1996)