Abstract

Using an integrated optics ring-resonator biosensor, we have demonstrated the detection of protein in low concentrations. We detected 0.3 nM of avidin in a buffered saline solution; the calculated detection limit is 0.1 nM (6.8ngml) for avidin, which compares favorably with those of other optical protein detection techniques. Further improvement is possible. Our ring resonator utilizes SixNySiO2 waveguides, which, owing to evanescent field interaction, change the effective refractive index when target molecules are immobilized on their surfaces. The selectivity of the sensor depends on the biotin surface coating, which causes the specific binding and immobilization of avidin.

© 2005 Optical Society of America

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References

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    [CrossRef]
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    [CrossRef] [PubMed]
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2004 (2)

N. T. Greene, S. L. Morgan, and K. D. Shimizu, Chem. Commun. 10, 1172 (2004).
[CrossRef]

A. Ksendzov, M. L. Homer, and A. M. Manfreda, Electron. Lett. 40, 63 (2004).
[CrossRef]

2003 (1)

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

2002 (4)

P. Rabiei, W. H. Steier, C. Zhang, and L. R. Dalton, J. Lightwave Technol. 20, 1968 (2002).
[CrossRef]

M. Henning, Sens. Mater. 14, 339 (2002).
[CrossRef]

E. Krioukov, D. J.W. Klunder, A. Driessen, J. Greve, and C. Otto, Opt. Lett. 27, 512 (2002).
[CrossRef]

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

1999 (1)

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

1997 (1)

R. Polzius, E. Diessel, F. F. Bier, and U. Bilitewski, Anal. Biochem. 248, 269 (1997).
[CrossRef] [PubMed]

1995 (1)

R. Karlsson and R. Stahlberg, Anal. Biochem. 288, 274 (1995).
[CrossRef]

1984 (1)

Ameliya, T.

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

Arnold, S.

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Bier, F. F.

R. Polzius, E. Diessel, F. F. Bier, and U. Bilitewski, Anal. Biochem. 248, 269 (1997).
[CrossRef] [PubMed]

Bilitewski, U.

R. Polzius, E. Diessel, F. F. Bier, and U. Bilitewski, Anal. Biochem. 248, 269 (1997).
[CrossRef] [PubMed]

Braun, D.

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Brosinger, F.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Dalton, L. R.

Diessel, E.

R. Polzius, E. Diessel, F. F. Bier, and U. Bilitewski, Anal. Biochem. 248, 269 (1997).
[CrossRef] [PubMed]

Driessen, A.

Ehrfeld, W.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Freimuth, H.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Greene, N. T.

N. T. Greene, S. L. Morgan, and K. D. Shimizu, Chem. Commun. 10, 1172 (2004).
[CrossRef]

Greve, J.

Henning, M.

M. Henning, Sens. Mater. 14, 339 (2002).
[CrossRef]

Homer, M. L.

A. Ksendzov, M. L. Homer, and A. M. Manfreda, Electron. Lett. 40, 63 (2004).
[CrossRef]

Huang, X.

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

Itoh, K.

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

Karlsson, R.

R. Karlsson and R. Stahlberg, Anal. Biochem. 288, 274 (1995).
[CrossRef]

Khoshsima, M.

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Klunder, D. J.W.

Knoll, W.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Krioukov, E.

Ksendzov, A.

A. Ksendzov, M. L. Homer, and A. M. Manfreda, Electron. Lett. 40, 63 (2004).
[CrossRef]

Lacher, M.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Libchaber, A.

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Lucosz, W.

Manfreda, A. M.

A. Ksendzov, M. L. Homer, and A. M. Manfreda, Electron. Lett. 40, 63 (2004).
[CrossRef]

Mittler-Neher, S.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Morgan, S. L.

N. T. Greene, S. L. Morgan, and K. D. Shimizu, Chem. Commun. 10, 1172 (2004).
[CrossRef]

Otto, C.

Polzius, R.

R. Polzius, E. Diessel, F. F. Bier, and U. Bilitewski, Anal. Biochem. 248, 269 (1997).
[CrossRef] [PubMed]

Rabiei, P.

Shimizu, K. D.

N. T. Greene, S. L. Morgan, and K. D. Shimizu, Chem. Commun. 10, 1172 (2004).
[CrossRef]

Stahlberg, R.

R. Karlsson and R. Stahlberg, Anal. Biochem. 288, 274 (1995).
[CrossRef]

Steier, W. H.

Teraoka, I.

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Tiefenthaler, K.

Tovar, G.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Vollmer, F.

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Weisser, M.

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Xu, Y.

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

Yimit, A.

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

Zhang, C.

Anal. Biochem. (2)

R. Karlsson and R. Stahlberg, Anal. Biochem. 288, 274 (1995).
[CrossRef]

R. Polzius, E. Diessel, F. F. Bier, and U. Bilitewski, Anal. Biochem. 248, 269 (1997).
[CrossRef] [PubMed]

Appl. Phys. Lett. (1)

F. Vollmer, D. Braun, A. Libchaber, M. Khoshsima, I. Teraoka, and S. Arnold, Appl. Phys. Lett. 80, 4057 (2002).
[CrossRef]

Biosens. Bioelectron. (1)

M. Weisser, G. Tovar, S. Mittler-Neher, W. Knoll, F. Brosinger, H. Freimuth, M. Lacher, and W. Ehrfeld, Biosens. Bioelectron. 14, 405 (1999).
[CrossRef] [PubMed]

Chem. Commun. (1)

N. T. Greene, S. L. Morgan, and K. D. Shimizu, Chem. Commun. 10, 1172 (2004).
[CrossRef]

Chem. Lett. (1)

A. Yimit, X. Huang, Y. Xu, T. Ameliya, and K. Itoh, Chem. Lett. 32, 86 (2003).
[CrossRef]

Electron. Lett. (1)

A. Ksendzov, M. L. Homer, and A. M. Manfreda, Electron. Lett. 40, 63 (2004).
[CrossRef]

J. Lightwave Technol. (1)

Opt. Lett. (2)

Sens. Mater. (1)

M. Henning, Sens. Mater. 14, 339 (2002).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

(a) Waveguide cross section, (b) ring-resonator layout, (c) signal taken from output A. 1 Å = 0.1 nm .

Fig. 2
Fig. 2

Schematic representation of the measurement setup.

Fig. 3
Fig. 3

Resonator peak position during flow of an avidin–PBS solution and of pure PBS. The sample flow sequence is marked. Buffer flow regions are numbered sequentially. Inset, sensor signal versus avidin concentration. See text for fit parameters.

Tables (1)

Tables Icon

Table 1 Signal Slopes ( ° C min ) for Various Stages of Our Experiment

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