Abstract

A spectroscopy system is developed for studying the two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) of epithelial tissue in backscattering geometry. Our findings show that TPEF signals from epithelial and underlying stromal layers exhibit different spectral characteristics, providing information on the biomorphology and biochemistry of tissue. The SHG signal serves as a sensitive indicator of collagen to separate the epithelial layer from underlying stroma. The polarization dependence of the SHG signal reveals a well-ordered orientation of collagen fibers in the stromal layer. The results demonstrate the potential of depth-resolved TPEF and SHG in determining the pathology of epithelial tissue.

© 2005 Optical Society of America

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References

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  1. N. Ramanujam, Neoplasia 2, 89 (2000).
    [Crossref] [PubMed]
  2. Y. Wu, P. Xi, J. Y. Qu, T. Cheung, and M. Yu, Opt. Express 12, 3218 (2004).
    [Crossref] [PubMed]
  3. Y. Wu, P. Xi, J. Y. Qu, T. Cheung, and M. Yu, Opt. Express 13, 382 (2005).
    [Crossref] [PubMed]
  4. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
    [Crossref] [PubMed]
  5. Y. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, Opt. Lett. 22, 1323 (1997).
    [Crossref]
  6. A. T. Yeh, N. Nassif, A. Zoumi, and B. J. Tromberg, Opt. Lett. 27, 2082 (2002).
    [Crossref]
  7. S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2881 (2002).
  8. P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
    [Crossref] [PubMed]
  9. T. Yasui, Y. Tohno, and T. Araki, J. Biomed. Opt. 9, 259 (2004).
    [Crossref] [PubMed]

2005 (1)

2004 (2)

2002 (3)

A. T. Yeh, N. Nassif, A. Zoumi, and B. J. Tromberg, Opt. Lett. 27, 2082 (2002).
[Crossref]

S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2881 (2002).

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

2000 (1)

N. Ramanujam, Neoplasia 2, 89 (2000).
[Crossref] [PubMed]

1997 (1)

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Alfano, R. R.

Araki, T.

T. Yasui, Y. Tohno, and T. Araki, J. Biomed. Opt. 9, 259 (2004).
[Crossref] [PubMed]

Cheung, T.

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Guo, Y.

Harris, D.

Heikal, A. A.

S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2881 (2002).

Ho, P. P.

Huang, S.

S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2881 (2002).

Kim, B. M.

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

Liu, F.

Nassif, N.

Qu, J. Y.

Ramanujam, N.

N. Ramanujam, Neoplasia 2, 89 (2000).
[Crossref] [PubMed]

Reiser, K. M.

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

Rubenchik, A. M.

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

Sacks, P.

Savage, H.

Schantz, S.

Silva, L. B. D.

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

Stoller, P.

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Tohno, Y.

T. Yasui, Y. Tohno, and T. Araki, J. Biomed. Opt. 9, 259 (2004).
[Crossref] [PubMed]

Tromberg, B. J.

Webb, W. W.

S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2881 (2002).

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Wu, Y.

Xi, P.

Yasui, T.

T. Yasui, Y. Tohno, and T. Araki, J. Biomed. Opt. 9, 259 (2004).
[Crossref] [PubMed]

Yeh, A. T.

Yu, M.

Zhadin, N.

Zoumi, A.

Biophys. J. (1)

S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2881 (2002).

J. Biomed. Opt. (2)

P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, L. B. D. Silva, P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. D. Silva, J. Biomed. Opt. 7, 205 (2002).
[Crossref] [PubMed]

T. Yasui, Y. Tohno, and T. Araki, J. Biomed. Opt. 9, 259 (2004).
[Crossref] [PubMed]

Neoplasia (1)

N. Ramanujam, Neoplasia 2, 89 (2000).
[Crossref] [PubMed]

Opt. Express (2)

Opt. Lett. (2)

Science (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

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Figures (4)

Fig. 1
Fig. 1

Left, excited TPEF and SHG signals of nonkeratinized oral tissue; Right, Masson-stained section, coloring collagen blue.

Fig. 2
Fig. 2

Normalized two- and single-photon excited fluorescence spectral signals. a, Epithelial layer; b, stromal layer; c, pure collagen.

Fig. 3
Fig. 3

a, Left, TPEF and SHG signals of esophageal tissue; right, Masson-stained section. b, Left, TPEF and SHG signals of colonic tissue; right, Masson-stained section.

Fig. 4
Fig. 4

Polarization-dependent SHG signals recorded from oral stroma.

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