Abstract

We characterize experimentally the influence of sample structure and beam focusing on signal level in third-harmonic generation (THG) microscopy. In the case of a homogeneous spherical sample, the dependence of the signal on the size of the sphere can be controlled by modifying the Rayleigh length of the excitation beam. More generally, the influence of excitation focusing on the signal depends on sample geometry, allowing one to highlight certain structures within a complex system. We illustrate this point by focusing-based contrast modulation in THG images of Drosophila embryos.

© 2005 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Signal epidetection in third-harmonic generation microscopy of turbid media

Delphine Débarre, Nicolas Olivier, and Emmanuel Beaurepaire
Opt. Express 15(14) 8913-8924 (2007)

Combined third-harmonic generation and four-wave mixing microscopy of tissues and embryos

Pierre Mahou, Nicolas Olivier, Guillaume Labroille, Louise Duloquin, Jean-Marc Sintes, Nadine Peyriéras, Renaud Legouis, Delphine Débarre, and Emmanuel Beaurepaire
Biomed. Opt. Express 2(10) 2837-2849 (2011)

Higher harmonic generation microscopy of in vitro cultured mammal oocytes and embryos

Cho-Shuen Hsieh, Shee-Uan Chen, Yen-Wei Lee, Yu-Shih Yang, and Chi-Kuang Sun
Opt. Express 16(15) 11574-11588 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription