Abstract

We report on optical levitation and manipulation of microscopic particles that are stuck on a glass surface with pulsed optical tweezers. An infrared pulse laser at 1.06??m was used to generate a large gradient force (up to 10?9?N) within a short duration (?45??s) that overcomes the adhesive interaction between the particles and the glass surface. Then a low-power continuous-wave diode laser at 785?nm was used to capture and manipulate the levitated particle. We have demonstrated that both stuck dielectric and biological micrometer-sized particles, including polystyrene beads, yeast cells, and Bacillus cereus bacteria, can be levitated and manipulated with this technique. We measured the single-pulse levitation efficiency for 2.0??m polystyrene beads as a function of the pulse energy and of the axial displacement from the stuck particle to the pulsed laser focus, which was as high as 88%.

© 2005 Optical Society of America

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    [CrossRef]
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  3. K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  7. B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
    [CrossRef]
  8. M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [PubMed]
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    [CrossRef]
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2004

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

B. Agate, C. T. Brown, W. Sibbett, and K. Dholakia, Opt. Express 12, 3011 (2004).
[CrossRef] [PubMed]

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

2003

D. G. Grier, Nature 424, 810 (2003).
[CrossRef] [PubMed]

C. Bustamante, Z. Bryant, and S. B. Smith, Nature 421, 423 (2003).
[CrossRef] [PubMed]

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

2002

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

C. A. Xie, M. A. Dinno, and Y.-Q. Li, Opt. Lett. 27, 249 (2002).
[CrossRef]

C. A. Xie and Y.-Q. Li, Appl. Phys. Lett. 81, 951 (2002).
[CrossRef]

1999

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

L. Schermelleh, S. Thalhammer, and W. Heckl, BioTechniques 27, 362 (1999).
[PubMed]

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

1986

Agate, B.

Arlt, J.

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Ashkin, A.

A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, Opt. Lett. 11, 288 (1986).
[CrossRef]

A. Ashkin, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 1–27.

Bergman, K.

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

Berns, M. W.

M. W. Berns, Y. Tadir, H. Liang, and B. Tromberg, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 71–98.

Bjorkholm, J. E.

Block, S. M.

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

Brown, C. T.

Bryant, Z.

C. Bustamante, Z. Bryant, and S. B. Smith, Nature 421, 423 (2003).
[CrossRef] [PubMed]

Bustamante, C.

C. Bustamante, Z. Bryant, and S. B. Smith, Nature 421, 423 (2003).
[CrossRef] [PubMed]

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

Cernohorska, H.

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

Chadd, E. H.

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

Chu, S.

Dholakia, K.

B. Agate, C. T. Brown, W. Sibbett, and K. Dholakia, Opt. Express 12, 3011 (2004).
[CrossRef] [PubMed]

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Dinno, M. A.

Dumont, S.

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

Dziedzic, J. M.

Grier, D. G.

D. G. Grier, Nature 424, 810 (2003).
[CrossRef] [PubMed]

Heckl, W.

L. Schermelleh, S. Thalhammer, and W. Heckl, BioTechniques 27, 362 (1999).
[PubMed]

Hobza, R.

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

Lengerova, M.

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

Li, Y.-Q.

C. A. Xie, M. A. Dinno, and Y.-Q. Li, Opt. Lett. 27, 249 (2002).
[CrossRef]

C. A. Xie and Y.-Q. Li, Appl. Phys. Lett. 81, 951 (2002).
[CrossRef]

Liang, H.

M. W. Berns, Y. Tadir, H. Liang, and B. Tromberg, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 71–98.

Liou, G. F.

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

Liphardt, J.

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

MacDonald, M. P.

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Mehta, A. D.

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

Neuman, K. C.

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

Onoa, B.

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

Peterson, L.

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Rief, M.

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

Rubes, J.

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

Schermelleh, L.

L. Schermelleh, S. Thalhammer, and W. Heckl, BioTechniques 27, 362 (1999).
[PubMed]

Sibbett, W.

B. Agate, C. T. Brown, W. Sibbett, and K. Dholakia, Opt. Express 12, 3011 (2004).
[CrossRef] [PubMed]

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Simmons, R. M.

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

Smith, D. A.

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

Smith, S. B.

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

C. Bustamante, Z. Bryant, and S. B. Smith, Nature 421, 423 (2003).
[CrossRef] [PubMed]

Spudich, J. A.

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

Tadir, Y.

M. W. Berns, Y. Tadir, H. Liang, and B. Tromberg, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 71–98.

Thalhammer, S.

L. Schermelleh, S. Thalhammer, and W. Heckl, BioTechniques 27, 362 (1999).
[PubMed]

Tinoco, I.

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

Tromberg, B.

M. W. Berns, Y. Tadir, H. Liang, and B. Tromberg, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 71–98.

Volke-Sepulveda, K.

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Vyskot, B.

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

Xie, C. A.

C. A. Xie, M. A. Dinno, and Y.-Q. Li, Opt. Lett. 27, 249 (2002).
[CrossRef]

C. A. Xie and Y.-Q. Li, Appl. Phys. Lett. 81, 951 (2002).
[CrossRef]

Appl. Phys. Lett.

C. A. Xie and Y.-Q. Li, Appl. Phys. Lett. 81, 951 (2002).
[CrossRef]

Biophys. J.

K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, Biophys. J. 77, 2856 (1999).
[CrossRef] [PubMed]

BioTechniques

L. Schermelleh, S. Thalhammer, and W. Heckl, BioTechniques 27, 362 (1999).
[PubMed]

Chromosome Res.

R. Hobza, M. Lengerova, H. Cernohorska, J. Rubes, and B. Vyskot, Chromosome Res. 12, 245 (2004).
[CrossRef]

Nature

D. G. Grier, Nature 424, 810 (2003).
[CrossRef] [PubMed]

C. Bustamante, Z. Bryant, and S. B. Smith, Nature 421, 423 (2003).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Rev. Sci. Instrum.

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

Science

A. D. Mehta, M. Rief, J. A. Spudich, D. A. Smith, and R. M. Simmons, Science 283, 1689 (1999).
[CrossRef] [PubMed]

B. Onoa, S. Dumont, J. Liphardt, S. B. Smith, I. Tinoco, and C. Bustamante, Science 292, 1892 (2003).
[CrossRef]

M. P. MacDonald, L. Peterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett, and K. Dholakia, Science 296, 1101 (2002).
[CrossRef] [PubMed]

Other

M. W. Berns, Y. Tadir, H. Liang, and B. Tromberg, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 71–98.

A. Ashkin, in Methods in Cell Biology, M. P. Sheetz, ed. (Academic, 1998), Vol. 55, pp. 1–27.

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