Abstract

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (?CCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a ?CCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the ?CCA devices daunorubicin accumulation is proportional to the liquid turnover rate.

© 2005 Optical Society of America

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  1. L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
    [CrossRef] [PubMed]
  2. A. Ghanem and M. L. Shuler, Biotechnol. Prog. 16, 334 (2000).
    [CrossRef] [PubMed]
  3. A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
    [CrossRef] [PubMed]
  4. K. Viravaidya, A. Sin, and M. L. Shuler, Biotechnol. Prog. 20, 316 (2004).
    [CrossRef] [PubMed]
  5. M. W. Davidson and M. Abramowitz, Optical Microscopy (Olympus America, 1999).
  6. A. Periasamy, ed., Methods in Cellular Imaging (Oxford U. Press, 2001).
  7. S. Landry, P. L. McGhee, R. J. Girardin, and W. J. Keeler, Opt. Express 12, 5754 (2004).
    [CrossRef] [PubMed]
  8. A. Laigle, M. M.L. Fiallo, and A. Garnier-Suillerot, Chem. Biol. Interact. 101, 49 (1996).
    [CrossRef] [PubMed]

2004 (3)

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

K. Viravaidya, A. Sin, and M. L. Shuler, Biotechnol. Prog. 20, 316 (2004).
[CrossRef] [PubMed]

S. Landry, P. L. McGhee, R. J. Girardin, and W. J. Keeler, Opt. Express 12, 5754 (2004).
[CrossRef] [PubMed]

2000 (1)

A. Ghanem and M. L. Shuler, Biotechnol. Prog. 16, 334 (2000).
[CrossRef] [PubMed]

1996 (1)

A. Laigle, M. M.L. Fiallo, and A. Garnier-Suillerot, Chem. Biol. Interact. 101, 49 (1996).
[CrossRef] [PubMed]

1995 (1)

L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
[CrossRef] [PubMed]

Abramowitz, M.

M. W. Davidson and M. Abramowitz, Optical Microscopy (Olympus America, 1999).

Babish, J. G.

L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
[CrossRef] [PubMed]

Chin, K. C.

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

Davidson, M. W.

M. W. Davidson and M. Abramowitz, Optical Microscopy (Olympus America, 1999).

Fiallo, M. M.L.

A. Laigle, M. M.L. Fiallo, and A. Garnier-Suillerot, Chem. Biol. Interact. 101, 49 (1996).
[CrossRef] [PubMed]

Garnier-Suillerot, A.

A. Laigle, M. M.L. Fiallo, and A. Garnier-Suillerot, Chem. Biol. Interact. 101, 49 (1996).
[CrossRef] [PubMed]

Ghanem, A.

A. Ghanem and M. L. Shuler, Biotechnol. Prog. 16, 334 (2000).
[CrossRef] [PubMed]

L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
[CrossRef] [PubMed]

Girardin, R. J.

Jamil, M. F.

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

Keeler, W. J.

Kostov, Y.

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

Laigle, A.

A. Laigle, M. M.L. Fiallo, and A. Garnier-Suillerot, Chem. Biol. Interact. 101, 49 (1996).
[CrossRef] [PubMed]

Landry, S.

McGhee, P. L.

Rao, G.

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

Shuler, M. L.

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

K. Viravaidya, A. Sin, and M. L. Shuler, Biotechnol. Prog. 20, 316 (2004).
[CrossRef] [PubMed]

A. Ghanem and M. L. Shuler, Biotechnol. Prog. 16, 334 (2000).
[CrossRef] [PubMed]

L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
[CrossRef] [PubMed]

Sin, A.

K. Viravaidya, A. Sin, and M. L. Shuler, Biotechnol. Prog. 20, 316 (2004).
[CrossRef] [PubMed]

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

Sweeney, L. M.

L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
[CrossRef] [PubMed]

Viravaidya, K.

K. Viravaidya, A. Sin, and M. L. Shuler, Biotechnol. Prog. 20, 316 (2004).
[CrossRef] [PubMed]

Biotechnol. Prog. (3)

A. Ghanem and M. L. Shuler, Biotechnol. Prog. 16, 334 (2000).
[CrossRef] [PubMed]

A. Sin, K. C. Chin, M. F. Jamil, Y. Kostov, G. Rao, and M. L. Shuler, Biotechnol. Prog. 20, 338 (2004).
[CrossRef] [PubMed]

K. Viravaidya, A. Sin, and M. L. Shuler, Biotechnol. Prog. 20, 316 (2004).
[CrossRef] [PubMed]

Chem. Biol. Interact. (1)

A. Laigle, M. M.L. Fiallo, and A. Garnier-Suillerot, Chem. Biol. Interact. 101, 49 (1996).
[CrossRef] [PubMed]

Opt. Express (1)

Toxicol. In Vitro (1)

L. M. Sweeney, M. L. Shuler, J. G. Babish, and A. Ghanem, Toxicol. In Vitro 9, 307 (1995).
[CrossRef] [PubMed]

Other (2)

M. W. Davidson and M. Abramowitz, Optical Microscopy (Olympus America, 1999).

A. Periasamy, ed., Methods in Cellular Imaging (Oxford U. Press, 2001).

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