Abstract

We report enhancement of two-photon fluorescence (TPF) excitation in fluorescent dyes and fluorescently labeled biomolecules by exploiting the optical properties of double grating waveguide structures (DGWSs). Picosecond laser pulses generate a large evanescent field based on the guided mode phenomenon in the resonant DGWSs, which induces strong TPF signals from fluorescent dyes at the waveguide surface. By recording enhanced TPF signals of Rhodamine B and Lucifer Yellow under resonance conditions, a detection sensitivity of concentrations of approximately one dye molecule per 0.1μm2 was achieved. For the first time to our knowledge, enhanced TPF signals of a Lucifer Yellow–labeled biomolecule (human self-peptide) in an aqueous environment are demonstrated. These results strongly encourage the use of DGWSs as enhancement platforms in modern biophysics and biotechnology for investigations of biological membranes and cells.

© 2005 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. J. R. Lakowicz and I. Gryczynski, in Vol. 5 of Topics in Fluorescence Spectroscopy (Plenum, 1997), pp. 87–122.
    [CrossRef]
  2. G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
    [CrossRef]
  3. C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
    [CrossRef]
  4. D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
    [CrossRef]
  5. D. Rosenblatt, A. Sharon, and A. A. Friesem, IEEE J. Quantum Electron. 33, 2038 (1999).
    [CrossRef]
  6. C. Kappel, A. Selle, M. A. Bader, and G. Marowsky, J. Opt. Soc. Am. B 21, 1127 (2004).
    [CrossRef]
  7. C. Xu and W. W. Webb, J. Opt. Soc. Am. B 13, 481 (1996).
    [CrossRef]
  8. W. W. Stewart, Nature 292, 17 (1981).
    [CrossRef] [PubMed]
  9. T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
    [CrossRef]
  10. U. Alexiev, I. Rimke, and T. Pöhlmann, J. Mol. Biol. 328, 705 (2003).
    [CrossRef] [PubMed]
  11. M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
    [CrossRef] [PubMed]

2004 (3)

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

C. Kappel, A. Selle, M. A. Bader, and G. Marowsky, J. Opt. Soc. Am. B 21, 1127 (2004).
[CrossRef]

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

2003 (2)

U. Alexiev, I. Rimke, and T. Pöhlmann, J. Mol. Biol. 328, 705 (2003).
[CrossRef] [PubMed]

D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
[CrossRef]

2001 (1)

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

1999 (1)

D. Rosenblatt, A. Sharon, and A. A. Friesem, IEEE J. Quantum Electron. 33, 2038 (1999).
[CrossRef]

1998 (1)

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

1996 (1)

1981 (1)

W. W. Stewart, Nature 292, 17 (1981).
[CrossRef] [PubMed]

Alexiev, U.

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

U. Alexiev, I. Rimke, and T. Pöhlmann, J. Mol. Biol. 328, 705 (2003).
[CrossRef] [PubMed]

Bader, M. A.

C. Kappel, A. Selle, M. A. Bader, and G. Marowsky, J. Opt. Soc. Am. B 21, 1127 (2004).
[CrossRef]

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Böckmann, R. A.

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

Bopp, M. A.

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Budach, W.

D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
[CrossRef]

Chibout, S.-D.

D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
[CrossRef]

Dupuis, M. L.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Duveneck, G. L.

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Ehrat, M.

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Fiorillo, M. T.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Fricke-Begemann, T.

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

Friesem, A. A.

D. Rosenblatt, A. Sharon, and A. A. Friesem, IEEE J. Quantum Electron. 33, 2038 (1999).
[CrossRef]

Greco, G.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Grubmüller, H.

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

Gryczynski, I.

J. R. Lakowicz and I. Gryczynski, in Vol. 5 of Topics in Fluorescence Spectroscopy (Plenum, 1997), pp. 87–122.
[CrossRef]

Haiml, M.

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Kappel, C.

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

C. Kappel, A. Selle, M. A. Bader, and G. Marowsky, J. Opt. Soc. Am. B 21, 1127 (2004).
[CrossRef]

Keller, U.

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Lakowicz, J. R.

J. R. Lakowicz and I. Gryczynski, in Vol. 5 of Topics in Fluorescence Spectroscopy (Plenum, 1997), pp. 87–122.
[CrossRef]

Maragno, M.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Marowsky, G.

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

C. Kappel, A. Selle, M. A. Bader, and G. Marowsky, J. Opt. Soc. Am. B 21, 1127 (2004).
[CrossRef]

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Monicio, A.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Neuschäfer, D.

D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
[CrossRef]

Pöhlmann, T.

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

U. Alexiev, I. Rimke, and T. Pöhlmann, J. Mol. Biol. 328, 705 (2003).
[CrossRef] [PubMed]

Potolicchio, I.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Rimke, I.

U. Alexiev, I. Rimke, and T. Pöhlmann, J. Mol. Biol. 328, 705 (2003).
[CrossRef] [PubMed]

Rosenblatt, D.

D. Rosenblatt, A. Sharon, and A. A. Friesem, IEEE J. Quantum Electron. 33, 2038 (1999).
[CrossRef]

Selle, A.

C. Kappel, A. Selle, M. A. Bader, and G. Marowsky, J. Opt. Soc. Am. B 21, 1127 (2004).
[CrossRef]

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

Sharon, A.

D. Rosenblatt, A. Sharon, and A. A. Friesem, IEEE J. Quantum Electron. 33, 2038 (1999).
[CrossRef]

Soria, S.

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Sorrentino, R.

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

Stewart, W. W.

W. W. Stewart, Nature 292, 17 (1981).
[CrossRef] [PubMed]

Uchanska-Ziegler, B.

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

Wanke, C.

D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
[CrossRef]

Webb, W. W.

Xu, C.

Ziegler, A.

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

Appl. Phys. B (1)

G. L. Duveneck, M. A. Bopp, M. Ehrat, M. Haiml, U. Keller, M. A. Bader, G. Marowsky, and S. Soria, Appl. Phys. B B73, 869 (2001).
[CrossRef]

Appl. Phys. B: Photophys. Laser Chem. (1)

C. Kappel, A. Selle, T. Fricke-Begemann, M. A. Bader, and G. Marowsky, Appl. Phys. B: Photophys. Laser Chem. 79, 531 (2004).
[CrossRef]

Biosens. Bioelectron. (1)

D. Neuschäfer, W. Budach, C. Wanke, and S.-D. Chibout, Biosens. Bioelectron. 18, 489 (2003).
[CrossRef]

Eur. J. Immunol. (1)

M. T. Fiorillo, G. Greco, M. Maragno, I. Potolicchio, A. Monicio, M. L. Dupuis, and R. Sorrentino, Eur. J. Immunol. 28, 2508 (1998).
[CrossRef] [PubMed]

IEEE J. Quantum Electron. (1)

D. Rosenblatt, A. Sharon, and A. A. Friesem, IEEE J. Quantum Electron. 33, 2038 (1999).
[CrossRef]

J. Biol. Chem. (1)

T. Pöhlmann, R. A. Böckmann, H. Grubmüller, B. Uchanska-Ziegler, A. Ziegler, and U. Alexiev, J. Biol. Chem. 279, 28197 (2004).
[CrossRef]

J. Mol. Biol. (1)

U. Alexiev, I. Rimke, and T. Pöhlmann, J. Mol. Biol. 328, 705 (2003).
[CrossRef] [PubMed]

J. Opt. Soc. Am. B (2)

Nature (1)

W. W. Stewart, Nature 292, 17 (1981).
[CrossRef] [PubMed]

Other (1)

J. R. Lakowicz and I. Gryczynski, in Vol. 5 of Topics in Fluorescence Spectroscopy (Plenum, 1997), pp. 87–122.
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1
Fig. 1

Experimental setup including a sketch of the DGWS sample (top left) and a CCD camera image under the resonance condition (bottom left).

Fig. 2
Fig. 2

Integrated CCD camera signal from 10 4 M LY induced by picosecond laser pulses as a function of the incident laser power, the second-order fit function, the linear-dependent part with a 1 = 0.0054 ( ± 0.0003 ) , and the square-dependent part with a 2 = 4.4 × 10 5 ( ± 1 × 10 6 ) .

Fig. 3
Fig. 3

Integrated CCD camera signals of 10 9 M LY (top) and 10 10 M LY (bottom) induced by picosecond laser pulses as a function of the incident laser power, the second-order fit function, and the linear- and square-dependent parts with a 1 = 0.0021 ( ± 0.0001 ) and a 2 = 2.1 × 10 6 ( ± 3 × 10 7 ) for 10 9 M LY and a 1 = 0.0025 ( ± 0.0001 ) and a 2 = 6 × 10 7 ( ± 3 × 10 7 ) for 10 10 M LY , respectively.

Fig. 4
Fig. 4

Integrated CCD camera signal of the 10 5 M LY-labeled human self-peptide induced by picosecond laser pulses as a function of the incident laser power, the second-order fit function, and the linear- and square-dependent parts with a 1 = 0.0011 ( ± 0.0003 ) and a 2 = 7.2 × 10 6 ( ± 6 × 10 7 ) , respectively. Inset, schematic representation of the DGWS sample in a quartz-window cuvette filled with water.

Metrics