Abstract

Surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques are integrated in a biosensing imaging system for measuring the two-dimensional spatial phase variation caused by biomolecular interactions on a sensing chip without the need for additional labeling. The common-path PSI technique has the advantage of long-term stability, even when it is subjected to external disturbances. Hence the system meets the requirements of the real-time kinetic studies involved in biomolecular interaction analysis. The proposed SPR–PSI imaging system demonstrates a detection limit of a 2×107 refractive-index change, a long-term phase stability of 2.5×104πrms for 4h, and a spatial phase resolution of 103π with a lateral resolution of 100μm.

© 2005 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. H. Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings (Springer-Verlag, Berlin, 1988).
  2. B. Liedberg, C. Nylander, and I. Lundsr, Sens. Actuators 4, 299 (1983).
    [CrossRef]
  3. J. Homola, S. S. Yee, and G. Gauglitz, Sens. Actuators B 54, 3 (1999).
    [CrossRef]
  4. C. E. Jordan and R. M. Corn, Anal. Chem. 69, 1449 (1997).
    [CrossRef] [PubMed]
  5. B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
    [CrossRef] [PubMed]
  6. A. V. Kabashin and P. I. Nikitin, Opt. Commun. 150, 5 (1998).
    [CrossRef]
  7. P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
    [CrossRef]
  8. A. G. Notcovich, V. Zhuk, and S. G. Lipson, Appl. Phys. Lett. 76, 1665 (2000).
    [CrossRef]
  9. M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
    [CrossRef]
  10. S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).
  11. P. Hariharan, B. F. Oreb, and T. Eiju, Appl. Opt. 26, 2504 (1987).
    [CrossRef] [PubMed]
  12. J.-J. Chyou, S.-J. Chen, and Y.-K. Chen, Appl. Opt. 43, 5655 (2004).
    [CrossRef] [PubMed]

2004 (1)

2001 (2)

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
[CrossRef]

2000 (2)

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

A. G. Notcovich, V. Zhuk, and S. G. Lipson, Appl. Phys. Lett. 76, 1665 (2000).
[CrossRef]

1999 (1)

J. Homola, S. S. Yee, and G. Gauglitz, Sens. Actuators B 54, 3 (1999).
[CrossRef]

1998 (1)

A. V. Kabashin and P. I. Nikitin, Opt. Commun. 150, 5 (1998).
[CrossRef]

1997 (1)

C. E. Jordan and R. M. Corn, Anal. Chem. 69, 1449 (1997).
[CrossRef] [PubMed]

1987 (1)

1983 (1)

B. Liedberg, C. Nylander, and I. Lundsr, Sens. Actuators 4, 299 (1983).
[CrossRef]

Beloglazov, A. A.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Brueck, S. R. J.

M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
[CrossRef]

Chen, S.-J.

J.-J. Chyou, S.-J. Chen, and Y.-K. Chen, Appl. Opt. 43, 5655 (2004).
[CrossRef] [PubMed]

S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).

Chen, Y.-K.

J.-J. Chyou, S.-J. Chen, and Y.-K. Chen, Appl. Opt. 43, 5655 (2004).
[CrossRef] [PubMed]

S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).

Chyou, J.-J.

Corn, R.

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

Corn, R. M.

C. E. Jordan and R. M. Corn, Anal. Chem. 69, 1449 (1997).
[CrossRef] [PubMed]

Eiju, T.

Gauglitz, G.

J. Homola, S. S. Yee, and G. Gauglitz, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Goodman, R. M.

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

Grigorenko, A. N.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Grimsrud, T. E.

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

Hariharan, P.

Homola, J.

J. Homola, S. S. Yee, and G. Gauglitz, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Hsiu, F.-M.

S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).

Huebner, A.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Jordan, C. E.

C. E. Jordan and R. M. Corn, Anal. Chem. 69, 1449 (1997).
[CrossRef] [PubMed]

Kabashin, A. V.

A. V. Kabashin and P. I. Nikitin, Opt. Commun. 150, 5 (1998).
[CrossRef]

Kuhne, C.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Liedberg, B.

B. Liedberg, C. Nylander, and I. Lundsr, Sens. Actuators 4, 299 (1983).
[CrossRef]

Lies, M. R.

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

Lipson, S. G.

A. G. Notcovich, V. Zhuk, and S. G. Lipson, Appl. Phys. Lett. 76, 1665 (2000).
[CrossRef]

Lopez, G. P.

M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
[CrossRef]

Lundsr, I.

B. Liedberg, C. Nylander, and I. Lundsr, Sens. Actuators 4, 299 (1983).
[CrossRef]

Nelson, B. P.

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

Nikitin, P. I.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

A. V. Kabashin and P. I. Nikitin, Opt. Commun. 150, 5 (1998).
[CrossRef]

Notcovich, A. G.

A. G. Notcovich, V. Zhuk, and S. G. Lipson, Appl. Phys. Lett. 76, 1665 (2000).
[CrossRef]

Nylander, C.

B. Liedberg, C. Nylander, and I. Lundsr, Sens. Actuators 4, 299 (1983).
[CrossRef]

O’Brien, M. J.

M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
[CrossRef]

Oreb, B. F.

Perez-Luna, V. H.

M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
[CrossRef]

Raether, H.

H. Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings (Springer-Verlag, Berlin, 1988).

Salzer, R.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Savchuk, A. I.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Savchuk, O. A.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Steiner, G.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Su, Y.-D.

S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).

Tsou, C.-Y.

S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).

Valeiko, M. V.

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Yee, S. S.

J. Homola, S. S. Yee, and G. Gauglitz, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Zhuk, V.

A. G. Notcovich, V. Zhuk, and S. G. Lipson, Appl. Phys. Lett. 76, 1665 (2000).
[CrossRef]

Anal. Chem. (2)

C. E. Jordan and R. M. Corn, Anal. Chem. 69, 1449 (1997).
[CrossRef] [PubMed]

B. P. Nelson, T. E. Grimsrud, M. R. Lies, R. M. Goodman, and R. Corn, Anal. Chem. 73, 1 (2001).
[CrossRef] [PubMed]

Appl. Opt. (2)

Appl. Phys. Lett. (1)

A. G. Notcovich, V. Zhuk, and S. G. Lipson, Appl. Phys. Lett. 76, 1665 (2000).
[CrossRef]

Biosens. Bioelectron. (1)

M. J. O’Brien, V. H. Perez-Luna, S. R. J. Brueck, and G. P. Lopez, Biosens. Bioelectron. 16, 97 (2001).
[CrossRef]

Opt. Commun. (1)

A. V. Kabashin and P. I. Nikitin, Opt. Commun. 150, 5 (1998).
[CrossRef]

Sens. Actuators (1)

B. Liedberg, C. Nylander, and I. Lundsr, Sens. Actuators 4, 299 (1983).
[CrossRef]

Sens. Actuators A (1)

P. I. Nikitin, A. N. Grigorenko, A. A. Beloglazov, M. V. Valeiko, A. I. Savchuk, O. A. Savchuk, G. Steiner, C. Kuhne, A. Huebner, and R. Salzer, Sens. Actuators A 85, 189 (2000).
[CrossRef]

Sens. Actuators B (1)

J. Homola, S. S. Yee, and G. Gauglitz, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Other (2)

S.-J. Chen, Y.-D. Su, F.-M. Hsiu, C.-Y. Tsou, and Y.-K. Chen, “Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis,” J. Biomed. Opt. (to be published).

H. Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings (Springer-Verlag, Berlin, 1988).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

System setup: 1, He–Ne laser; 2, 13, 15, linear polarizers; 3, beam expander with spatial filter; 4, prism; 5, Cr; 6, Au; 7, sample layer; 8, flow cell; 9, TE cooler; 10, liquid-crystal device; 11, image lens; 12, beam splitter; 14, CCD camera; 16, photodiode.

Fig. 2
Fig. 2

Long-term phase stability of 2.5 × 10 4 π rms for 4 h .

Fig. 3
Fig. 3

(a) Interference patterns at five phase-modulator axis angles in a 50 × 50   pixel region covering a 1.0 × 1.0 mm physical area. (b) Spatial distribution of the phase shift recovered from a DNA microarray. (c) Spatial phase variation of a line cut from (b).

Metrics