Abstract

Surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques are integrated in a biosensing imaging system for measuring the two-dimensional spatial phase variation caused by biomolecular interactions on a sensing chip without the need for additional labeling. The common-path PSI technique has the advantage of long-term stability, even when it is subjected to external disturbances. Hence the system meets the requirements of the real-time kinetic studies involved in biomolecular interaction analysis. The proposed SPR–PSI imaging system demonstrates a detection limit of a 2×107 refractive-index change, a long-term phase stability of 2.5×104πrms for 4h, and a spatial phase resolution of 103π with a lateral resolution of 100μm.

© 2005 Optical Society of America

Full Article  |  PDF Article
Related Articles
Characteristics of a paired surface plasma waves biosensor

Chien Chou, Hsieh-Ting Wu, Yen-Chen Huang, Yi-Ling Chen, and Wen-Chuan Kuo
Opt. Express 14(10) 4307-4315 (2006)

Side-polished multimode fiber biosensor based on surface plasmon resonance with halogen light

Hong-Yu Lin, Woo-Hu Tsai, Yu-Chia Tsao, and Bor-Chiou Sheu
Appl. Opt. 46(5) 800-806 (2007)

Two-dimensional biosensor arrays based on surface plasmon resonance phase imaging

C. L. Wong, H. P. Ho, T. T. Yu, Y. K. Suen, Winnie W. Y. Chow, S. Y. Wu, W. C. Law, W. Yuan, W. J. Li, S. K. Kong, and Chinlon Lin
Appl. Opt. 46(12) 2325-2332 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription