Abstract

We present a new technique that produces en face tomographic images with a 10-μs acquisition time per image. The setup consists of an interference microscope with stroboscopic illumination provided by a xenon arc flash lamp (10-μs flashes at 15Hz). The tomographic images are obtained from two phase-opposed interferometric images recorded simultaneously by two synchronized CCD cameras. Transverse resolution better than 1.0μm is achieved by use of high-numerical-aperture microscope objectives. The short coherence length of the source yields an axial resolution of 0.9μm. 3×3  pixel binning leads to a detection sensitivity of 71dB. Our system is suitable for various applications, particularly in biology for in vivo cellular-level imaging.

© 2005 Optical Society of America

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