Abstract

Poor axial precision caused, in part, by large depth of focus (τ) has been a vexing problem in extraction of particle position from digital in-line holograms. A simple method is proposed to combat this depth-of-focus difficulty. The method is based on decoupling of size and position information. With d,Δ, and λ being particle diameter, CCD pixel size, and the wavelength, respectively, our main theoretical result is the reduction of τ from τd2λ to τΔ2λ for particles of known size. This result is confirmed in laboratory experiments with holograms of calibrated glass spheres.

© 2005 Optical Society of America

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