Abstract

We present a new type of flow cytometer that can operate underwater for a long time, as long as days, for measuring the size distribution, concentration, and biomass of marine phytoplankton. The major improvement of the instrument over existing techniques is the elimination of sample preparation, which is achieved with a laser Doppler crossed-beam arrangement for both defining a measurement volume and measuring the speed of the particle traversing it. By simultaneously sampling the laser-induced fluorescence signal and the Doppler signals, the technique can discriminate sizes of phytoplankton.

© 2005 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
    [CrossRef] [PubMed]
  2. J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
    [CrossRef] [PubMed]
  3. R. K.Y. Chan and Ka Man Un, Appl. Opt. 40, 2956 (2001).
    [CrossRef]
  4. A. Desiderio, C. Moore, C. Lantz, and T. J. Cowles, Appl. Opt. 36, 1289 (1997).
    [CrossRef] [PubMed]
  5. S. G. Ackleson, R. W. Spinrad, C. M. Yentsch, J. F. Brown, and W. Korjeff-Bellows, Appl. Opt. 27, 1262 (1988).
    [CrossRef] [PubMed]
  6. L. E. Drain, The Laser Doppler Technique (Wiley, New York, 1980).
  7. A. Radbruch, Flow Cytometry and Cell Sorting (Springer-Verlag, Berlin, 1992).
    [CrossRef]
  8. Y. Yeh and H. Z. Cummins, Appl. Phys. Lett. 4, 176 (1964).
    [CrossRef]
  9. P. G. Falkowski and Z. Kolber, Aust. J. Plant Physiol. 22, 341 (1995).
    [CrossRef]
  10. U. Schreiber and A. Krieger, FEBS Lett. 397, 131 (1996).
    [CrossRef] [PubMed]

2001 (1)

1999 (2)

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
[CrossRef] [PubMed]

1997 (1)

1996 (1)

U. Schreiber and A. Krieger, FEBS Lett. 397, 131 (1996).
[CrossRef] [PubMed]

1995 (1)

P. G. Falkowski and Z. Kolber, Aust. J. Plant Physiol. 22, 341 (1995).
[CrossRef]

1988 (1)

1964 (1)

Y. Yeh and H. Z. Cummins, Appl. Phys. Lett. 4, 176 (1964).
[CrossRef]

Ackleson, S. G.

Baethmann, A.

J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
[CrossRef] [PubMed]

Beeker, A. E.R.

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

Brown, J. F.

Chan, R. K.Y.

Cowles, T. J.

Cummins, H. Z.

Y. Yeh and H. Z. Cummins, Appl. Phys. Lett. 4, 176 (1964).
[CrossRef]

Desiderio, A.

Drain, L. E.

L. E. Drain, The Laser Doppler Technique (Wiley, New York, 1980).

Dubelaar, G. B.J.

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

Falkowski, P. G.

P. G. Falkowski and Z. Kolber, Aust. J. Plant Physiol. 22, 341 (1995).
[CrossRef]

Gerritzen, P. L.

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

Jonker, R. R.

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

Kachel, V.

J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
[CrossRef] [PubMed]

Kolber, Z.

P. G. Falkowski and Z. Kolber, Aust. J. Plant Physiol. 22, 341 (1995).
[CrossRef]

Korjeff-Bellows, W.

Krieger, A.

U. Schreiber and A. Krieger, FEBS Lett. 397, 131 (1996).
[CrossRef] [PubMed]

Lantz, C.

Moore, C.

Plesnila, N.

J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
[CrossRef] [PubMed]

Radbruch, A.

A. Radbruch, Flow Cytometry and Cell Sorting (Springer-Verlag, Berlin, 1992).
[CrossRef]

Schreiber, U.

U. Schreiber and A. Krieger, FEBS Lett. 397, 131 (1996).
[CrossRef] [PubMed]

Spinrad, R. W.

Tangen, K.

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

Un, Ka Man

Wietzorrek, J.

J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
[CrossRef] [PubMed]

Yeh, Y.

Y. Yeh and H. Z. Cummins, Appl. Phys. Lett. 4, 176 (1964).
[CrossRef]

Yentsch, C. M.

Appl. Opt. (3)

Appl. Phys. Lett. (1)

Y. Yeh and H. Z. Cummins, Appl. Phys. Lett. 4, 176 (1964).
[CrossRef]

Aust. J. Plant Physiol. (1)

P. G. Falkowski and Z. Kolber, Aust. J. Plant Physiol. 22, 341 (1995).
[CrossRef]

Cytometry (2)

G. B.J. Dubelaar, P. L. Gerritzen, A. E.R. Beeker, R. R. Jonker, and K. Tangen, Cytometry 37, 247 (1999).
[CrossRef] [PubMed]

J. Wietzorrek, N. Plesnila, A. Baethmann, and V. Kachel, Cytometry 35, 291 (1999).
[CrossRef] [PubMed]

FEBS Lett. (1)

U. Schreiber and A. Krieger, FEBS Lett. 397, 131 (1996).
[CrossRef] [PubMed]

Other (2)

L. E. Drain, The Laser Doppler Technique (Wiley, New York, 1980).

A. Radbruch, Flow Cytometry and Cell Sorting (Springer-Verlag, Berlin, 1992).
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (5)

Fig. 1
Fig. 1

Optical probe setup: PMT, photomultiplier tube.

Fig. 2
Fig. 2

Bow-tie-shaped measurement volume.

Fig. 3
Fig. 3

Synchronous Doppler burst with a LIF signal and fast Fourier transform (FFT). LDV, laser Doppler velocimetry.

Fig. 4
Fig. 4

Size distribution measurement.

Fig. 5
Fig. 5

Temporal concentration measurement.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

l = λ 2 n sin ( α 2 ) ,
f = 2 V n sin ( α 2 ) λ ,
D = V t W .

Metrics