Abstract

A method that combines fluorescence imaging and spectroscopy of single molecules at room temperature is presented. This approach allows us to identify a number of imaged molecules unequivocally by simultaneously recording their fluorescence emission spectra. Furthermore, their spectral characteristics not only allow us to separate different fluorescent labels quantitatively and qualitatively but also provide information on the microenvironment of the molecules. This new method was successfully tested on a system of yellow-green and red fluorescent 20-nm latex beads, and its usefulness in studies of biological systems was illustrated for a preparation of combined binary ratio labeling–fluorescence in situ hybridization-stained mouse chromosomes.

© 2004 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Integrated endoscopy system for simultaneous imaging and spectroscopy for early lung cancer detection

Haishan Zeng, Mirjan Petek, Marjeta Tercelj Zorman, Annette McWilliams, Branko Palcic, and Stephen Lam
Opt. Lett. 29(6) 587-589 (2004)

Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy

Jenifer F. Ojeda, Changan Xie, Yong-Qing Li, Fred E. Bertrand, John Wiley, and Thomas J. McConnell
Opt. Express 14(12) 5385-5393 (2006)

Luminescent images of single gold nanoparticles and their labeling on silica beads

Jian Zhang, Yi Fu, and Joseph R. Lakowicz
Opt. Express 15(20) 13415-13420 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription