Abstract

A novel approach to reconstructing both the absorption and the scattering properties of a turbid medium simultaneously from steady-state broadband spectral measurements is presented that utilizes second-differential fitting to the water spectrum to estimate the optical path length in tissue. Theoretical and experimental evidence is provided to demonstrate the robust accuracy of the spectroscopy approach and reconstructed absorption images. The steady-state broadband CCD system has the potential to provide accurate chromophore imaging without the technological complexity of time- or frequency-domain systems.

© 2004 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. S. R. Arridge and W. R. B. Lionheart, Opt. Lett. 23, 882 (1998).
    [CrossRef]
  2. A. Corlu, T. Durduran, R. Choe, M. Schweiger, E. M. C. Hillman, S. R. Arridge, and A. G. Yodh, Opt. Lett. 28, 2339 (2003).
    [CrossRef] [PubMed]
  3. A. Li, Q. Zhang, J. P. Culver, E. L. Miller, and D. A. Boas, Opt. Lett. 29, 256 (2004).
    [CrossRef] [PubMed]
  4. H. Jiang, K. D. Paulsen, U. L. Osterberg, B. W. Pogue, and M. S. Patterson, J. Opt. Soc. Am. A 13, 253 (1996).
    [CrossRef]
  5. S. R. Arridge, M. Cope, and D. T. Delpy, Phys. Med. Biol. 37, 1531 (1992).
    [CrossRef] [PubMed]
  6. S. J. Matcher, M. Cope, and D. T. Delpy, Phys. Med. Biol. 39, 177 (1994).
    [CrossRef] [PubMed]
  7. H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
    [CrossRef]
  8. R. F. Reinoso, B. A. Telfer, and M. Rowland, J. Pharmacol. Toxicol. Methods 38, 87 (1997).
    [CrossRef] [PubMed]
  9. B. L. Horecker, J. Biol. Chem. 148, 173 (1943).
  10. V. Hollis, “Noninvasive monitoring of brain-tissue temperature by near-infrared spectroscopy,” Ph.D. dissertation (University College, London, 2002).
  11. H. J. van Staveren, C. J. M. Moses, J. van Marle, S. A. Prahl, and M. J. C. van Gemert, Appl. Opt. 30, 4507 (1991).
    [CrossRef] [PubMed]

2004 (1)

2003 (2)

A. Corlu, T. Durduran, R. Choe, M. Schweiger, E. M. C. Hillman, S. R. Arridge, and A. G. Yodh, Opt. Lett. 28, 2339 (2003).
[CrossRef] [PubMed]

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

1998 (1)

1997 (1)

R. F. Reinoso, B. A. Telfer, and M. Rowland, J. Pharmacol. Toxicol. Methods 38, 87 (1997).
[CrossRef] [PubMed]

1996 (1)

1994 (1)

S. J. Matcher, M. Cope, and D. T. Delpy, Phys. Med. Biol. 39, 177 (1994).
[CrossRef] [PubMed]

1992 (1)

S. R. Arridge, M. Cope, and D. T. Delpy, Phys. Med. Biol. 37, 1531 (1992).
[CrossRef] [PubMed]

1991 (1)

1943 (1)

B. L. Horecker, J. Biol. Chem. 148, 173 (1943).

Arridge, S. R.

Boas, D. A.

Choe, R.

Cope, M.

S. J. Matcher, M. Cope, and D. T. Delpy, Phys. Med. Biol. 39, 177 (1994).
[CrossRef] [PubMed]

S. R. Arridge, M. Cope, and D. T. Delpy, Phys. Med. Biol. 37, 1531 (1992).
[CrossRef] [PubMed]

Corlu, A.

Culver, J. P.

Dehghani, H.

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

Delpy, D. T.

S. J. Matcher, M. Cope, and D. T. Delpy, Phys. Med. Biol. 39, 177 (1994).
[CrossRef] [PubMed]

S. R. Arridge, M. Cope, and D. T. Delpy, Phys. Med. Biol. 37, 1531 (1992).
[CrossRef] [PubMed]

Dunn, J. F.

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

Durduran, T.

Hillman, E. M. C.

Hollis, V.

V. Hollis, “Noninvasive monitoring of brain-tissue temperature by near-infrared spectroscopy,” Ph.D. dissertation (University College, London, 2002).

Horecker, B. L.

B. L. Horecker, J. Biol. Chem. 148, 173 (1943).

Jiang, H.

Li, A.

Lionheart, W. R. B.

Matcher, S. J.

S. J. Matcher, M. Cope, and D. T. Delpy, Phys. Med. Biol. 39, 177 (1994).
[CrossRef] [PubMed]

Miller, E. L.

Moses, C. J. M.

Osterberg, U. L.

Patterson, M. S.

Paulsen, K. D.

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

H. Jiang, K. D. Paulsen, U. L. Osterberg, B. W. Pogue, and M. S. Patterson, J. Opt. Soc. Am. A 13, 253 (1996).
[CrossRef]

Pogue, B. W.

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

H. Jiang, K. D. Paulsen, U. L. Osterberg, B. W. Pogue, and M. S. Patterson, J. Opt. Soc. Am. A 13, 253 (1996).
[CrossRef]

Prahl, S. A.

Reinoso, R. F.

R. F. Reinoso, B. A. Telfer, and M. Rowland, J. Pharmacol. Toxicol. Methods 38, 87 (1997).
[CrossRef] [PubMed]

Rowland, M.

R. F. Reinoso, B. A. Telfer, and M. Rowland, J. Pharmacol. Toxicol. Methods 38, 87 (1997).
[CrossRef] [PubMed]

Schweiger, M.

Springett, R.

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

Telfer, B. A.

R. F. Reinoso, B. A. Telfer, and M. Rowland, J. Pharmacol. Toxicol. Methods 38, 87 (1997).
[CrossRef] [PubMed]

van Gemert, M. J. C.

van Marle, J.

van Staveren, H. J.

Xu, H.

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

Yodh, A. G.

Zhang, Q.

Appl. Opt. (1)

J. Biol. Chem. (1)

B. L. Horecker, J. Biol. Chem. 148, 173 (1943).

J. Opt. Soc. Am. A (1)

J. Pharmacol. Toxicol. Methods (1)

R. F. Reinoso, B. A. Telfer, and M. Rowland, J. Pharmacol. Toxicol. Methods 38, 87 (1997).
[CrossRef] [PubMed]

Opt. Lett. (3)

Phys. Med. Biol. (2)

S. R. Arridge, M. Cope, and D. T. Delpy, Phys. Med. Biol. 37, 1531 (1992).
[CrossRef] [PubMed]

S. J. Matcher, M. Cope, and D. T. Delpy, Phys. Med. Biol. 39, 177 (1994).
[CrossRef] [PubMed]

Proc. SPIE (1)

H. Xu, B. W. Pogue, H. Dehghani, R. Springett, K. D. Paulsen, and J. F. Dunn, Proc. SPIE 4955, 16 (2003).
[CrossRef]

Other (1)

V. Hollis, “Noninvasive monitoring of brain-tissue temperature by near-infrared spectroscopy,” Ph.D. dissertation (University College, London, 2002).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1
Fig. 1

Left, Diagram of the multichannel broadband full-spectral CCD system. Right, photograph of the system with the filter wheels and the source wheel at the left and the source and the spectrometer at the right.

Fig. 2
Fig. 2

Predicted (dashed curves) versus reconstructed (solid curves) values for μa and μs when both the Intralipid and the HbO2 concentrations are varied: Left, μa versus HbO2 concentration; right, μs versus Intralipid concentration.

Fig. 3
Fig. 3

Left, theoretical desired image with a sample reconstructed set of images. In the cross-section plots (μa on the left and μs on the right) are shown for three such phantom tests. Scales are normalized by the background values.

Fig. 4
Fig. 4

Examples of reconstructed tomography images of a rat head in normal and hypoxic states are shown. Left, μa image (740 nm) at the normal state with the inspired oxygen fraction of 30%. Middle, μa of the hypoxic state when the fraction dropped to 10%. The increase in absorption indicated the increased Hb concentration and the decreased saturation. Right, MRI image acquired simultaneously with the NIR measurement, provided the fiber positions for image reconstruction.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

d2Adλ2=Aμad2μadλ2+Aμsd2μsdλ2+dA/μadλdμaλ+dA/μsdλdμsλ.
d2Adλ2Aμad2μadλ2.
d2Adλ2=i=1NcDPCid2idλ2,

Metrics