Abstract

We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue.

© 2004 Optical Society of America

Full Article  |  PDF Article
Related Articles
Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup

Ruggero Micheletto, Morgan Denyer, Martin Scholl, Ken Nakajima, Andreas Offenhauser, Masahiko Hara, and Wolfgang Knoll
Appl. Opt. 38(31) 6648-6652 (1999)

Influence of semicrystalline order on the second-harmonic generation efficiency in the anisotropic bands of myocytes

Catherine Greenhalgh, Nicole Prent, Chantal Green, Richard Cisek, Arkady Major, Bryan Stewart, and Virginijus Barzda
Appl. Opt. 46(10) 1852-1859 (2007)

Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy

François Tiaho, Gaëlle Recher, and Denis Rouède
Opt. Express 15(19) 12286-12295 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription