Abstract

We describe a method for en face phase-contrast imaging of cells with a fiber-based differential phase-contrast optical coherence microscopy system. Recorded en face images are quantitative phase-contrast maps of cells due to spatial variation of the refractive index and (or) thickness of various cellular components. Quantitative phase-contrast images of human epithelial cheek cells obtained with the fiber-based differential phase-contrast optical coherence microscopy system are presented.

© 2004 Optical Society of America

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2002 (1)

2001 (2)

C. Yang, A. Wax, R. R. Dasari, and M. S. Feld, Opt. Lett. 26, 686 (2001).
[CrossRef]

D. P. Davé, T. Akkin, T. E. Milner, and H. Grady Rylander, Opt. Commun. 193, 39 (2001).
[CrossRef]

2000 (3)

1999 (1)

1997 (1)

E. B. Van Munster, L. J. Van Vliet, and J. A. Aten, J. Microsc. 188, 149 (1997).
[CrossRef]

1994 (1)

Akkin, T.

D. P. Davé, T. Akkin, T. E. Milner, and H. Grady Rylander, Opt. Commun. 193, 39 (2001).
[CrossRef]

Aten, J. A.

E. B. Van Munster, L. J. Van Vliet, and J. A. Aten, J. Microsc. 188, 149 (1997).
[CrossRef]

Badizadegan, K.

Barty, A.

A. Barty, K. A. Nugent, A. Roberts, and D. Paganin, Opt. Commun. 175, 329 (2000).
[CrossRef]

Bevilacqua, F.

Cuche, E.

Dasari, R. R.

Davé, D. P.

D. P. Davé, T. Akkin, T. E. Milner, and H. Grady Rylander, Opt. Commun. 193, 39 (2001).
[CrossRef]

D. P. Davé and T. E. Milner, Opt. Lett. 25, 227 (2000).
[CrossRef]

Despeursinge, C.

Feld, M. S.

Fercher, A. F.

Georgakoudi, I.

Gotzinger, E.

Grady Rylander, H.

D. P. Davé, T. Akkin, T. E. Milner, and H. Grady Rylander, Opt. Commun. 193, 39 (2001).
[CrossRef]

Hanlon, E. B.

Hitzenberger, C. K.

Milner, T. E.

D. P. Davé, T. Akkin, T. E. Milner, and H. Grady Rylander, Opt. Commun. 193, 39 (2001).
[CrossRef]

D. P. Davé and T. E. Milner, Opt. Lett. 25, 227 (2000).
[CrossRef]

Nugent, K. A.

A. Barty, K. A. Nugent, A. Roberts, and D. Paganin, Opt. Commun. 175, 329 (2000).
[CrossRef]

Paganin, D.

A. Barty, K. A. Nugent, A. Roberts, and D. Paganin, Opt. Commun. 175, 329 (2000).
[CrossRef]

Pircher, M.

Roberts, A.

A. Barty, K. A. Nugent, A. Roberts, and D. Paganin, Opt. Commun. 175, 329 (2000).
[CrossRef]

Sattmann, H.

Sticker, M.

Van Munster, E. B.

E. B. Van Munster, L. J. Van Vliet, and J. A. Aten, J. Microsc. 188, 149 (1997).
[CrossRef]

Van Vliet, L. J.

E. B. Van Munster, L. J. Van Vliet, and J. A. Aten, J. Microsc. 188, 149 (1997).
[CrossRef]

Wax, A.

Yang, C.

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Figures (4)

Fig. 1
Fig. 1

Height profile of the chromium nanostep measured with the prototype DPC-OCM system and an atomic force microscope (AFM).

Fig. 2
Fig. 2

(a) Sample path configuration for recording bright-field and en face DPC-OCM images. (b) Lateral and (c) collinear configurations of sample path optics for spatially separating probe beams. The optical axis ( or ) of the birefringent elements in the lateral and collinear configurations is indicated.

Fig. 3
Fig. 3

A, B DPC-OCM and C, D bright-field microscope images of single human epithelial cheek cells. A, Lateral and B, collinear probe beam configurations were used to record DPC-OCM images. Gray scale denotes single-pass OPL difference in nanometers solid scale bar=10 µm.

Fig. 4
Fig. 4

A, DPC-OCM and B, phase-contrast microscope image of multiple human epithelial cheek cells. The images are of similar epithelial cells but not the same sample. Gray scale denotes single-pass OPL difference in nanometers solid scale bar=20 µm.

Equations (5)

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Imz=2IoRrefRsampz1/2exp-z-zo/lc2×cos2πfot+φm+φnoise,
Δφ=φ2-φ1=22πλoΔp.
Δp=Δnldl.
Δφx,yo=4πλo0zonx+Δx,y0,z-nx,yo,zdz=4πΔxλo0zonx,yo,zxdz.
Δφx,yo=4πλo0zonx,yo,zdz-nglasstglass,

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