Abstract

We demonstrate the operation of a digital in-line microscope with LED illumination. We show with a practical example that, for typical setups, the limited temporal coherence and the spatial incoherence of the source do not affect the resolving power of the system. On the contrary, important advantages are obtained in terms of signal-to-noise ratio and alignment simplification.

© 2004 Optical Society of America

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References

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2003 (1)

2002 (2)

2001 (5)

H. J. Kreuzer, M. H. Jericho, I. A. Meinertzhagen, and W. Xu, J. Phys. Condens. Matter 13, 10729 (2001).
[CrossRef]

S. Coetmellec, C. Buraga-Lefebvre, C. Lebrun, and C. Ozcul, Meas. Sci. Technol. 12, 1392 (2001).
[CrossRef]

G. Indebetouw and P. Klysubun, J. Opt. Soc. Am. A 18, 319 (2001).
[CrossRef]

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).
[CrossRef]

S. Grilli, P. Ferraro, S. De Nicola, A. Finizio, G. Pierattini, and R. Meucci, Opt. Express 9, 294 (2001), http://www.opticsexpress.org .
[CrossRef] [PubMed]

2000 (1)

1999 (2)

E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 38, 6995 (1999).
[CrossRef]

F. Dubois, L. Joannes, and J. Legros, Appl. Opt. 38, 7085 (1999).
[CrossRef]

1996 (1)

U. Schnars, T. M. Kreis, and W. P. O. Jüptner, Opt. Eng. 35, 977 (1996).
[CrossRef]

1993 (1)

Anderson, W. L.

Buraga-Lefebvre, C.

S. Coetmellec, C. Buraga-Lefebvre, C. Lebrun, and C. Ozcul, Meas. Sci. Technol. 12, 1392 (2001).
[CrossRef]

Cathey, W. T.

W. T. Cathey, Optical Information Processing and Holography (Wiley, New York, 1974), pp. 314–363.

Coetmellec, S.

S. Coetmellec, C. Buraga-Lefebvre, C. Lebrun, and C. Ozcul, Meas. Sci. Technol. 12, 1392 (2001).
[CrossRef]

Cuche, E.

E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 38, 6995 (1999).
[CrossRef]

De Nicola, S.

Depeursinge, C.

E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 38, 6995 (1999).
[CrossRef]

Dubois, F.

Ferraro, P.

Finizio, A.

Goodman, J. W.

J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, New York, 1968), Chap. 8.

Grilli, S.

Hussain, F.

Indebetouw, G.

Jericho, M. H.

Joannes, L.

Jüptner, W. P. O.

U. Schnars and W. P. O. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

U. Schnars, T. M. Kreis, and W. P. O. Jüptner, Opt. Eng. 35, 977 (1996).
[CrossRef]

Klysubun, P.

Kreis, T. M.

U. Schnars, T. M. Kreis, and W. P. O. Jüptner, Opt. Eng. 35, 977 (1996).
[CrossRef]

Kreuzer, H. J.

Lebrun, C.

S. Coetmellec, C. Buraga-Lefebvre, C. Lebrun, and C. Ozcul, Meas. Sci. Technol. 12, 1392 (2001).
[CrossRef]

Legros, J.

Liu, D. D.

Marquet, P.

E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 38, 6995 (1999).
[CrossRef]

Massig, J. H.

Meinertzhagen, I. A.

Meng, H.

Meucci, R.

Ozcul, C.

S. Coetmellec, C. Buraga-Lefebvre, C. Lebrun, and C. Ozcul, Meas. Sci. Technol. 12, 1392 (2001).
[CrossRef]

Pedrini, G.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).
[CrossRef]

Pierattini, G.

Schedin, S.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).
[CrossRef]

Schnars, U.

U. Schnars and W. P. O. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

U. Schnars, T. M. Kreis, and W. P. O. Jüptner, Opt. Eng. 35, 977 (1996).
[CrossRef]

Stadelmeier, A.

Tiziani, H. J.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).
[CrossRef]

Xu, W.

Appl. Opt. (3)

J. Mod. Opt. (1)

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).
[CrossRef]

J. Opt. Soc. Am. A (2)

J. Phys. Condens. Matter (1)

H. J. Kreuzer, M. H. Jericho, I. A. Meinertzhagen, and W. Xu, J. Phys. Condens. Matter 13, 10729 (2001).
[CrossRef]

Meas. Sci. Technol. (2)

S. Coetmellec, C. Buraga-Lefebvre, C. Lebrun, and C. Ozcul, Meas. Sci. Technol. 12, 1392 (2001).
[CrossRef]

U. Schnars and W. P. O. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

Opt. Eng. (1)

U. Schnars, T. M. Kreis, and W. P. O. Jüptner, Opt. Eng. 35, 977 (1996).
[CrossRef]

Opt. Express (1)

Opt. Lett. (2)

Other (2)

W. T. Cathey, Optical Information Processing and Holography (Wiley, New York, 1974), pp. 314–363.

J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, New York, 1968), Chap. 8.

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Figures (2)

Fig. 1
Fig. 1

Setup for a lensless digital holographic microscope with LED illumination.

Fig. 2
Fig. 2

Two different samples of 10µm latex spheres on a glass slide. (a), (d) Holograms generated with a green LED and digitally reconstructed in (b) and (e). (c), (f) Images obtained with a Nikon Eclipse TE300 inverted microscope (CFI Plan Fluor 60×, 0.85-N.A. objective).

Equations (5)

Equations on this page are rendered with MathJax. Learn more.

Z=LzL-z
M=Lz.
UZX,Y=HxH,yHPZX-xH,Y-yHdSH,
PZX,Y=expX2+Y2+Z21/2,
δx=λL-zW=2.5 µm,

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