Abstract
We report in vivo imaging of neuronal electrical activity from superficial layers of the mouse barrel cortex. The measurements have spatial and 3-ms temporal resolution and reach depths of below the cortical surface. The depth-dependent differential-fluorescence optical sections of activity are consistent with known cortical architecture and represent an important step toward in vivo measurement of functioning complex neural networks. Our observations employ a custom gradient-index lens probe and voltage-sensitive dye fluorescence; the use of epi-illumination rather than dark-field illumination provides the dramatic signal-to-noise improvement necessary for fast three-dimensional imaging.
© 2004 Optical Society of America
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