Abstract

We report in vivo imaging of neuronal electrical activity from superficial layers of the mouse barrel cortex. The measurements have 16µm spatial and 3-ms temporal resolution and reach depths of 150 µm below the cortical surface. The depth-dependent differential-fluorescence optical sections of activity are consistent with known cortical architecture and represent an important step toward in vivo measurement of functioning complex neural networks. Our observations employ a custom gradient-index lens probe and voltage-sensitive dye fluorescence; the use of epi-illumination rather than dark-field illumination provides the dramatic signal-to-noise improvement necessary for fast three-dimensional imaging.

© 2004 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Depth-dependent cerebral hemodynamic responses following Direct Cortical Electrical Stimulation (DCES) revealed by in vivo dual-optical imaging techniques

Seungduk Lee, Dalkwon Koh, Areum Jo, Hae Young Lim, Young-Jin Jung, Choong-Ki Kim, Youngwook Seo, Chang-Hwan Im, Beop-Min Kim, and Minah Suh
Opt. Express 20(7) 6932-6943 (2012)

Imaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes

Vassiliy Tsytsarev, Kaushalya Premachandra, Daisuke Takeshita, and Sonya Bahar
Opt. Lett. 33(9) 1032-1034 (2008)

Laminar optical tomography of the hemodynamic response in the lumbar spinal cord of rats

Nicolas Ouakli, Edgar Guevara, Simon Dubeau, Éric Beaumont, and Frédéric Lesage
Opt. Express 18(10) 10068-10077 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription