Abstract

A novel technique for the calibration of laser trapping systems that utilizes two-photon-excited fluorescence of commercial dye-stained microspheres has been demonstrated. The trapping forces as well as the trapping efficiency have been measured for various liquid environments and trapping depths. The trapping efficiency in water was found to decrease with an increase of trapping depths because of the enlargement of the trapping beam waist caused by aberrations of the optical system.

© 2003 Optical Society of America

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  1. S. Sato, M. Ohyumi, H. Shibata, H. Hinaba, and Y. Ogawa, Opt. Lett. 16, 282 (1991).
    [CrossRef] [PubMed]
  2. K. Svoboda and S. B. Block, Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
    [CrossRef]
  3. W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Opt. 33, 1735 (1994).
    [CrossRef] [PubMed]
  4. H. Felgner, O. Muller, and M. Schliwa, Appl. Opt. 34, 977 (1995).
    [CrossRef] [PubMed]
  5. M. E. J. Friese, H. Rubinsztein-Dunlop, N. R. Heckenberg, and E. W. Dearden, Appl. Opt. 35, 7112 (1996).
    [CrossRef] [PubMed]
  6. E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
    [CrossRef]
  7. S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
    [CrossRef]
  8. W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
    [CrossRef]
  9. N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
    [CrossRef]
  10. Y. Liu, G. J. Sonek, M. W. Berns, K. Konig, and B. J. Tromberg, Opt. Lett. 20, 2246 (1995).
    [CrossRef]
  11. P. N. Prasad, Introduction to Biophotonics (Wiley, New York, 2003).
    [CrossRef]
  12. W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Phys. Lett. 63, 715 (1993).
    [CrossRef]

2002 (1)

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
[CrossRef]

2000 (1)

W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
[CrossRef]

1999 (1)

S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
[CrossRef]

1998 (1)

E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
[CrossRef]

1996 (1)

1995 (2)

1994 (2)

K. Svoboda and S. B. Block, Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef]

W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Opt. 33, 1735 (1994).
[CrossRef] [PubMed]

1993 (1)

W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Phys. Lett. 63, 715 (1993).
[CrossRef]

1991 (1)

Arimondo, E.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
[CrossRef]

Bernet, S.

W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
[CrossRef]

Berns, M. W.

Block, S. B.

K. Svoboda and S. B. Block, Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef]

Dearden, E. W.

Felgner, H.

Florin, E. L.

E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
[CrossRef]

Friese, M. E. J.

Gallet, F.

S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
[CrossRef]

Heckenberg, N. R.

Hecker, N.

W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
[CrossRef]

Hénon, S.

S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
[CrossRef]

Hinaba, H.

Horber, J. K. H.

E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
[CrossRef]

Konig, K.

Lenormand, G.

S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
[CrossRef]

Liu, Y.

Malagnino, N.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
[CrossRef]

Muller, O.

Ogawa, Y.

Ohyumi, M.

Pesce, G.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
[CrossRef]

Pralle, A.

E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
[CrossRef]

Prasad, P. N.

P. N. Prasad, Introduction to Biophotonics (Wiley, New York, 2003).
[CrossRef]

Richert, A.

S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
[CrossRef]

Ritsch-Marte, M.

W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
[CrossRef]

Rubinsztein-Dunlop, H.

Sasso, A.

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
[CrossRef]

Sato, S.

Schliwa, M.

Shibata, H.

Singer, W.

W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
[CrossRef]

Sonek, G. J.

Stelzer, E. H. K.

E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
[CrossRef]

Svoboda, K.

K. Svoboda and S. B. Block, Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef]

Tromberg, B. J.

Wright, W. H.

W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Opt. 33, 1735 (1994).
[CrossRef] [PubMed]

W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Phys. Lett. 63, 715 (1993).
[CrossRef]

Annu. Rev. Biophys. Biomol. Struct. (1)

K. Svoboda and S. B. Block, Annu. Rev. Biophys. Biomol. Struct. 23, 247 (1994).
[CrossRef]

Appl. Opt. (3)

Appl. Phys. A (1)

E. L. Florin, A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, Appl. Phys. A 66, S75 (1998).
[CrossRef]

Appl. Phys. Lett. (1)

W. H. Wright, G. J. Sonek, and M. W. Berns, Appl. Phys. Lett. 63, 715 (1993).
[CrossRef]

Biophys. J. (1)

S. Hénon, G. Lenormand, A. Richert, and F. Gallet, Biophys. J. 76, 1145 (1999).
[CrossRef]

J. Mod. Opt. (1)

W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, J. Mod. Opt. 47, 2921 (2000).
[CrossRef]

Opt. Commun. (1)

N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, Opt. Commun. 214, 15 (2002).
[CrossRef]

Opt. Lett. (2)

Other (1)

P. N. Prasad, Introduction to Biophotonics (Wiley, New York, 2003).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

Trapping forces: (a) diagram of transverse forces acting on a trapped bead during trap rotation in a liquid environment; (b) trapping forces versus trapping laser power measured for fluorescent beads of several diameters.

Fig. 2
Fig. 2

Trapping efficiency: (a) its dependence on bead diameter, (b) dependence of Q/β on trap depth for various liquids; the viscosity of liquids is shown in centipoises (cp). The magnitude of the experimental error is comparable with the size of the symbols.

Fig. 3
Fig. 3

Trapping stiffness for 1 and 2.5 beads plotted versus trapping laser power.

Tables (1)

Tables Icon

Table 1 Refractive Index and Viscosity of Liquids Used for Experimentsa

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

Ftrap=6π2dνrβη2+πd2ρν1621/2,
Ftrap6π2dνrβη,
Q=FtrapcnP=6π2dνrβηcnP,

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