Abstract

Optical tomography of turbid media has so far been limited by systems that require fixed geometries or measurements employing fibers. We present a system that records noncontact optical measurements from diffuse media of arbitrary shapes and retrieves the three-dimensional surface information of the diffuse medium. We further present a novel method of combining this composite data set and obtain accurate fluorescence reconstructions. This approach offers significant experimental simplicity and yields high-information-content datasets. The performance of this novel tomographic approach is demonstrated with experimental reconstructions of phantoms.

© 2003 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. Biomedical Topical Meetings, Vol. 71 of OSA Trends in Optics and Photonics Series (Optical Society of America, Washington, D.C., 2002).
  2. J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
    [CrossRef] [PubMed]
  3. A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
    [CrossRef] [PubMed]
  4. J. Ripoll, M. Nieto-Vesperinas, R. Weissleder, and V. Ntziachristos, Opt. Lett. 27, 527 (2002).
    [CrossRef]
  5. M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
    [CrossRef] [PubMed]
  6. V. Ntziachristos and R. Weissleder, Opt. Lett. 26, 893 (2001).
    [CrossRef]
  7. M. A. Oleary, D. A. Boas, B. Chance, and A. G. Yodh, Opt. Lett. 20, 428 (1995).
  8. E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003).
    [CrossRef] [PubMed]

2003 (1)

E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003).
[CrossRef] [PubMed]

2002 (3)

Biomedical Topical Meetings, Vol. 71 of OSA Trends in Optics and Photonics Series (Optical Society of America, Washington, D.C., 2002).

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

J. Ripoll, M. Nieto-Vesperinas, R. Weissleder, and V. Ntziachristos, Opt. Lett. 27, 527 (2002).
[CrossRef]

2001 (1)

1996 (1)

M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
[CrossRef] [PubMed]

1995 (1)

M. A. Oleary, D. A. Boas, B. Chance, and A. G. Yodh, Opt. Lett. 20, 428 (1995).

Abdoulaev, G.

A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
[CrossRef] [PubMed]

Arridge, S.

M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
[CrossRef] [PubMed]

Arridge, S. R.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Austin, T.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Barbour, R. L.

A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
[CrossRef] [PubMed]

Bluestone, A. Y.

A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
[CrossRef] [PubMed]

Boas, D. A.

M. A. Oleary, D. A. Boas, B. Chance, and A. G. Yodh, Opt. Lett. 20, 428 (1995).

Chance, B.

M. A. Oleary, D. A. Boas, B. Chance, and A. G. Yodh, Opt. Lett. 20, 428 (1995).

Delpy, D.

M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
[CrossRef] [PubMed]

Delpy, D. T.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Everdell, N.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Firbank, M.

M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
[CrossRef] [PubMed]

Gibson, A.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Graves, E.

E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003).
[CrossRef] [PubMed]

Hebden, J. C.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Hielscher, A. H.

A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
[CrossRef] [PubMed]

Hillman, E. M. C.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Meek, J. H.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Nieto-Vesperinas, M.

Ntziachristos, V.

Oleary, M. A.

M. A. Oleary, D. A. Boas, B. Chance, and A. G. Yodh, Opt. Lett. 20, 428 (1995).

Ripoll, J.

E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003).
[CrossRef] [PubMed]

J. Ripoll, M. Nieto-Vesperinas, R. Weissleder, and V. Ntziachristos, Opt. Lett. 27, 527 (2002).
[CrossRef]

Schmitz, C. H.

A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
[CrossRef] [PubMed]

Schweiger, M.

M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
[CrossRef] [PubMed]

Weissleder, R.

Wyatt, J. S.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Yodh, A. G.

M. A. Oleary, D. A. Boas, B. Chance, and A. G. Yodh, Opt. Lett. 20, 428 (1995).

Yusof, R. M.

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

Med. Phys. (1)

E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003).
[CrossRef] [PubMed]

Opt. Express (1)

A. Y. Bluestone, G. Abdoulaev, C. H. Schmitz, R. L. Barbour, and A. H. Hielscher, Opt. Express 9, 272 (2001), http://www.opticsexpress.org.
[CrossRef] [PubMed]

Opt. Lett. (3)

OSA Trends in Optics and Photonics Series (1)

Biomedical Topical Meetings, Vol. 71 of OSA Trends in Optics and Photonics Series (Optical Society of America, Washington, D.C., 2002).

Phys. Med. Biol. (2)

J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, and J. S. Wyatt, Phys. Med. Biol. 47, 4155 (2002).
[CrossRef] [PubMed]

M. Firbank, S. Arridge, M. Schweiger, and D. Delpy, Phys. Med. Biol. 41, 767 (1996).
[CrossRef] [PubMed]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

Top view of the experimental setup showing the conventional CCD camera and the surface imaging camera employed. The inset shows the resin phantom imaged in this study.

Fig. 2
Fig. 2

Axial phantom slices at position y=0.26 cm taken from the reconstructed 3D mesh. All images shown here are interpolated five times from the original set. (a), (b) Reconstruction obtained for (a) both tubes containing the same concentration of Cy5.5 dye (1 µM) and (b) for 0.5µM Cy5.5 on the right tube and 1µM Cy5.5 on the left tube. (c) Reconstruction performed with the exact solution for slab geometry. The outline of the phantom is shown by a white curve. The dye concentration was 1 µM in both tubes, as in (a).

Fig. 3
Fig. 3

3D volume renderings of results obtained (total of 6481 voxels reconstructed and 10,020 surface elements). The volume rendered ranges over 1-cm length. The renderings are for (a) both tubes containing 1 µM of Cy5.5 dye and (b) the dye in the right tube replaced by 0.5µM dye solution.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

Udrs,rd=gκrs-rd-14πSgκr-rdn+1CndDgκr+rd×Jnrs,rdSr, rdV,
Jdrs,rd=1πSJnrs,rΓr,rddSr, rdV,
Γr,rd=ξr,rdfr-rdr-rd2cosθ cosθdΔA.

Metrics