Abstract

The combination of optical coherence tomography and spectroscopy may allow for highly localized, quantitative measurements of tissue spectral properties. We present, for the first time to our knowledge, quantitative measurements of the absorption coefficients of phantoms and of hemoglobin and oxygenated hemoglobin with spectroscopic optical coherence tomography (SOCT). Our results suggest that SOCT will be able to provide localized, quantitative oxygenation measurements.

© 2003 Optical Society of America

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References

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  1. J. A. Izatt, M. D. Kulkarni, S. Yazdanfar, J. K. Barton, and A. J. Welch, Opt. Lett. 22, 1439 (1997).
    [CrossRef]
  2. J. F. de Boer, T. E. Milner, M. J. C. van Gemert, and J. S. Nelson, Opt. Lett. 22, 934 (1997).
    [CrossRef] [PubMed]
  3. M. D. Kulkarni and J. A. Izatt, in Conference on Lasers and Electro Optics, Vol. 9 of 1996 OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1996), pp. 59–60.
  4. J. M. Schmitt, S. H. Xiang, and K. M. Yung, J. Opt. Soc. Am. A 15, 2288 (1998).
    [CrossRef]
  5. U. Morgner, W. Drexler, F. X. Kärtner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, Opt. Lett. 25, 111 (2000).
    [CrossRef]
  6. R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, Opt. Lett. 25, 820 (2000).
    [CrossRef]
  7. M. Siegemund, J. van Bommel, and C. Ince, Intensive Care Med. 25, 1044 (1999).
    [CrossRef] [PubMed]
  8. S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
    [CrossRef] [PubMed]
  9. T. G. van Leeuwen, M. D. Kulkarni, S. Yazdanfar, and J. A. Izatt, in Biomedical Optics, OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1999).
  10. A. M. Rollins, M. D. Kulkarni, S. Yazdanfar, R. Ung-arunyawee, and J. A. Izatt, Opt. Express 3, 219 (1998), http://www.opticsexpress.org.
    [CrossRef] [PubMed]
  11. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
    [CrossRef] [PubMed]
  12. T. G. van Leeuwen, D. J. Faber, and M. C. Aalders, “Measurement of the axial point spread function in scattering media using single mode fiber based optical coherence tomography,” IEEE J. Sel. Top. Quantum Electron. (to be published).

2000 (2)

1999 (1)

M. Siegemund, J. van Bommel, and C. Ince, Intensive Care Med. 25, 1044 (1999).
[CrossRef] [PubMed]

1998 (2)

1997 (2)

1994 (1)

1988 (1)

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

Aalders, M. C.

T. G. van Leeuwen, D. J. Faber, and M. C. Aalders, “Measurement of the axial point spread function in scattering media using single mode fiber based optical coherence tomography,” IEEE J. Sel. Top. Quantum Electron. (to be published).

Barton, J. K.

Cope, M.

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

de Boer, J. F.

Delpy, D. T.

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

Drexler, W.

Faber, D. J.

T. G. van Leeuwen, D. J. Faber, and M. C. Aalders, “Measurement of the axial point spread function in scattering media using single mode fiber based optical coherence tomography,” IEEE J. Sel. Top. Quantum Electron. (to be published).

Fercher, A. F.

Fujimoto, J. G.

Hee, M. R.

Hitzenberger, C. K.

Ince, C.

M. Siegemund, J. van Bommel, and C. Ince, Intensive Care Med. 25, 1044 (1999).
[CrossRef] [PubMed]

Ippen, E. P.

Izatt, J. A.

A. M. Rollins, M. D. Kulkarni, S. Yazdanfar, R. Ung-arunyawee, and J. A. Izatt, Opt. Express 3, 219 (1998), http://www.opticsexpress.org.
[CrossRef] [PubMed]

J. A. Izatt, M. D. Kulkarni, S. Yazdanfar, J. K. Barton, and A. J. Welch, Opt. Lett. 22, 1439 (1997).
[CrossRef]

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
[CrossRef] [PubMed]

T. G. van Leeuwen, M. D. Kulkarni, S. Yazdanfar, and J. A. Izatt, in Biomedical Optics, OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1999).

M. D. Kulkarni and J. A. Izatt, in Conference on Lasers and Electro Optics, Vol. 9 of 1996 OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1996), pp. 59–60.

Kärtner, F. X.

Kowalczyk, A.

Kulkarni, M. D.

A. M. Rollins, M. D. Kulkarni, S. Yazdanfar, R. Ung-arunyawee, and J. A. Izatt, Opt. Express 3, 219 (1998), http://www.opticsexpress.org.
[CrossRef] [PubMed]

J. A. Izatt, M. D. Kulkarni, S. Yazdanfar, J. K. Barton, and A. J. Welch, Opt. Lett. 22, 1439 (1997).
[CrossRef]

M. D. Kulkarni and J. A. Izatt, in Conference on Lasers and Electro Optics, Vol. 9 of 1996 OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1996), pp. 59–60.

T. G. van Leeuwen, M. D. Kulkarni, S. Yazdanfar, and J. A. Izatt, in Biomedical Optics, OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1999).

Leitgeb, R.

Li, X. D.

Milner, T. E.

Morgner, U.

Nelson, J. S.

Owen, G. M.

Pitris, C.

Reynolds, E. O. R.

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

Rollins, A. M.

Schmitt, J. M.

J. M. Schmitt, S. H. Xiang, and K. M. Yung, J. Opt. Soc. Am. A 15, 2288 (1998).
[CrossRef]

Siegemund, M.

M. Siegemund, J. van Bommel, and C. Ince, Intensive Care Med. 25, 1044 (1999).
[CrossRef] [PubMed]

Sticker, M.

Swanson, E. A.

Ung-arunyawee, R.

van Bommel, J.

M. Siegemund, J. van Bommel, and C. Ince, Intensive Care Med. 25, 1044 (1999).
[CrossRef] [PubMed]

van Gemert, M. J. C.

van Leeuwen, T. G.

T. G. van Leeuwen, M. D. Kulkarni, S. Yazdanfar, and J. A. Izatt, in Biomedical Optics, OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1999).

T. G. van Leeuwen, D. J. Faber, and M. C. Aalders, “Measurement of the axial point spread function in scattering media using single mode fiber based optical coherence tomography,” IEEE J. Sel. Top. Quantum Electron. (to be published).

Welch, A. J.

Wojtkowski, M.

Wray, S.

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

Wyatt, J. S.

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

Xiang, S. H.

J. M. Schmitt, S. H. Xiang, and K. M. Yung, J. Opt. Soc. Am. A 15, 2288 (1998).
[CrossRef]

Yazdanfar, S.

Yung, K. M.

J. M. Schmitt, S. H. Xiang, and K. M. Yung, J. Opt. Soc. Am. A 15, 2288 (1998).
[CrossRef]

1996 OSA Technical Digest Series (1)

M. D. Kulkarni and J. A. Izatt, in Conference on Lasers and Electro Optics, Vol. 9 of 1996 OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1996), pp. 59–60.

Biochim. Biophys. Acta (1)

S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochim. Biophys. Acta 933, 184 (1988).
[CrossRef] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

T. G. van Leeuwen, D. J. Faber, and M. C. Aalders, “Measurement of the axial point spread function in scattering media using single mode fiber based optical coherence tomography,” IEEE J. Sel. Top. Quantum Electron. (to be published).

Intensive Care Med. (1)

M. Siegemund, J. van Bommel, and C. Ince, Intensive Care Med. 25, 1044 (1999).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

J. M. Schmitt, S. H. Xiang, and K. M. Yung, J. Opt. Soc. Am. A 15, 2288 (1998).
[CrossRef]

Opt. Express (1)

Opt. Lett. (5)

OSA Technical Digest Series (1)

T. G. van Leeuwen, M. D. Kulkarni, S. Yazdanfar, and J. A. Izatt, in Biomedical Optics, OSA Technical Digest Series (Optical Society of America, Washington, D.C., 1999).

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Figures (3)

Fig. 1
Fig. 1

Absorption spectra of Hb and HbO2 plotted using tabulated values of the molar extinction coefficient cm-1/M of Hb and HbO2 (Ref. 8) and a concentration value of 150 g/L and 66500 g/mol. The bold curve represents the spectrum of the Ti:sapphire laser used in the experiments.

Fig. 2
Fig. 2

Measured absorption coefficient of a glass filter (Maico πc13) measured by SOCT. The gray curve represents the absorption spectrum from spectrometer measurements. Spectral resolution was 1 nm. Data are the average of 1000 absorption spectra, and the error bars correspond to the standard deviation (for clarity, not all error bars are plotted).

Fig. 3
Fig. 3

Absorption spectra of Hb and HbO2 as measured by SOCT. Spectral resolution was 1 nm. The plotted curves are the average of 1000 absorption spectra; the error bars correspond to the standard deviation (for clarity, not all error bars are plotted).

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

Iω=SiiωHω,
μaλ=-1dlnI2λI1λ.
lSTFT=π4 ln 2lcN,

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