Abstract

A novel optical heterodyne surface-plasmon resonance (SPR) biosensor with a Zeeman laser is proposed. Two surface plasma waves are excited by two correlated p-polarized waves in a SPR device of the Kretschmann configuration. Two reflected p waves are optically heterodyned such that the magnitude of the heterodyned signal is proportional to the multiplication of two attenuated reflected p waves. Then the detection sensitivity and the dynamic range based on this amplitude-sensitive method are enhanced. In the experiment, the kinetics between mouse immunoglobulin G (IgG) and rabbit antimouse IgG is obtained from sensograms of various concentrations of antimouse IgG. A detection sensitivity of 0.2 nM was achieved. In addition, a concentration of 5 ng/ml of protein G interacting with mouse IgG was measured successfully.

© 2003 Optical Society of America

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  1. D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
    [CrossRef] [PubMed]
  2. W. Lukosz, Biosens. Bioelectron. 6, 215 (1991).
    [CrossRef]
  3. A. Brandenburg, R. Krauter, C. Künzel, M. Stefan, and H. Schulte, Appl. Opt. 39, 6396 (2000).
    [CrossRef]
  4. S. Shen, T. Lin, and J. Guo, Appl. Opt. 37, 1747 (1998).
    [CrossRef]
  5. W. C. Kuo and C. Chou, in Proceedings of Optics and Photonics Taiwan '01 (Institute of Electro-Optical Engineering, National Sun Yat-Sen University, Taiwan, 2001), Vol. 1, p. 325.
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    [CrossRef]
  7. A. Kolomenskii, P. Gershon, and H. Schussler, Appl. Opt. 36, 6539 (1997).
    [CrossRef]
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    [CrossRef]
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    [CrossRef]
  11. IASYS application note 2.1, http://www.affinity_sensors.com.
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    [CrossRef] [PubMed]

2001 (1)

W. C. Kuo and C. Chou, in Proceedings of Optics and Photonics Taiwan '01 (Institute of Electro-Optical Engineering, National Sun Yat-Sen University, Taiwan, 2001), Vol. 1, p. 325.

2000 (2)

1999 (1)

J. Homola and S. S. Yee, Sens. Actuators B 54, 3 (1999).
[CrossRef]

1998 (2)

S. Shen, T. Lin, and J. Guo, Appl. Opt. 37, 1747 (1998).
[CrossRef]

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

1997 (1)

1995 (1)

D. Yeung, A. Gill, C. H. Maule, and R. J. Davies, Trends Anal. Chem. 14, 49 (1995).

1993 (1)

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

1992 (1)

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

1991 (1)

W. Lukosz, Biosens. Bioelectron. 6, 215 (1991).
[CrossRef]

Brandenburg, A.

Brighma-Burke, M.

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

Brooks, I.

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

Chou, C.

W. C. Kuo and C. Chou, in Proceedings of Optics and Photonics Taiwan '01 (Institute of Electro-Optical Engineering, National Sun Yat-Sen University, Taiwan, 2001), Vol. 1, p. 325.

Davies, R. J.

D. Yeung, A. Gill, C. H. Maule, and R. J. Davies, Trends Anal. Chem. 14, 49 (1995).

Davis, T. M.

T. M. Davis and W. D. Widson, Anal. Biochem. 284, 348 (2000).
[CrossRef] [PubMed]

Fägerstam, L. G.

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

Fleminger, G.

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

Frostell-Karlsson, Å.

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

Gershon, P.

Gill, A.

D. Yeung, A. Gill, C. H. Maule, and R. J. Davies, Trends Anal. Chem. 14, 49 (1995).

Guo, J.

Hensley, P.

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

Homola, J.

J. Homola and S. S. Yee, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Ivnitski, D.

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

Karlsson, R.

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

Kolomenskii, A.

Krauter, R.

Künzel, C.

Kuo, W. C.

W. C. Kuo and C. Chou, in Proceedings of Optics and Photonics Taiwan '01 (Institute of Electro-Optical Engineering, National Sun Yat-Sen University, Taiwan, 2001), Vol. 1, p. 325.

Lin, T.

Lukosz, W.

W. Lukosz, Biosens. Bioelectron. 6, 215 (1991).
[CrossRef]

Maule, C. H.

D. Yeung, A. Gill, C. H. Maule, and R. J. Davies, Trends Anal. Chem. 14, 49 (1995).

O'Shannessy, D. J.

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

Persson, B.

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

Rishpon, J.

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

Roh¨nnberg, I.

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

Schulte, H.

Schussler, H.

Shen, S.

Solomon, B.

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

Soneson, K. K.

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

Stefan, M.

Widson, W. D.

T. M. Davis and W. D. Widson, Anal. Biochem. 284, 348 (2000).
[CrossRef] [PubMed]

Wolf, T.

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

Yee, S. S.

J. Homola and S. S. Yee, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Yeung, D.

D. Yeung, A. Gill, C. H. Maule, and R. J. Davies, Trends Anal. Chem. 14, 49 (1995).

Anal. Biochem. (2)

D. J. O'Shannessy, M. Brighma-Burke, K. K. Soneson, P. Hensley, and I. Brooks, Anal. Biochem. 212, 457 (1993).
[CrossRef] [PubMed]

T. M. Davis and W. D. Widson, Anal. Biochem. 284, 348 (2000).
[CrossRef] [PubMed]

Appl. Opt. (3)

Bioelectrochem. Bioenerg. (1)

D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, and J. Rishpon, Bioelectrochem. Bioenerg. 45, 27 (1998).
[CrossRef]

Biosens. Bioelectron. (1)

W. Lukosz, Biosens. Bioelectron. 6, 215 (1991).
[CrossRef]

J. Chromatogr. (1)

L. G. Fägerstam, Å. Frostell-Karlsson, R. Karlsson, B. Persson, and I. Rőnnberg, J. Chromatogr. 597, 397 (1992).
[CrossRef]

Sens. Actuators B (1)

J. Homola and S. S. Yee, Sens. Actuators B 54, 3 (1999).
[CrossRef]

Trends Anal. Chem. (1)

D. Yeung, A. Gill, C. H. Maule, and R. J. Davies, Trends Anal. Chem. 14, 49 (1995).

Other (2)

W. C. Kuo and C. Chou, in Proceedings of Optics and Photonics Taiwan '01 (Institute of Electro-Optical Engineering, National Sun Yat-Sen University, Taiwan, 2001), Vol. 1, p. 325.

IASYS application note 2.1, http://www.affinity_sensors.com.

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Figures (5)

Fig. 1
Fig. 1

SPR sensor in the Kretchmann configuration. SAM, self-assembled monolayer.

Fig. 2
Fig. 2

Schematic of the optical heterodyne SPR biosensor: ZL, Zeeman He–Ne laser; Dref, detector; other abbreviations defined in text.

Fig. 3
Fig. 3

Detection sensitivity of one-SPW and two-SPW configurations (data are normalized to n3=1.254).

Fig. 4
Fig. 4

Growing curve of protein G interacting with mouse IgG.

Fig. 5
Fig. 5

Dynamic range of antimouse IgG interacting with mouse IgG in several concentrations [ng/ml].

Equations (9)

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ksp=ωc121+21/2,
kx=ω/c01/2 sin θsp=ksp.
Rθ=r04θ2= ri,i+1θ+ri+1,4θexp2jdi+1kz,i+1θ1+ri,i+1θri+1,4θexp2jdi+1kz,i+1θ2i=0,1,2,3,4.
Ip1+p2Δωt=½Ap2+½As2+ApAs cosΔωt,
Ip1+p2Δωt=½Ap2+½As2+ApAs cosΔωt,
Δdmin2spwΔdmin1spwrp21/2ωsrs11/2ωs<1,
Δdmin2spwΔdminIΔυΔW1/2<1,
Δkd=ωcdΔnc+ncωcΔd.
Δdeff=deffncΔnc+deffdΔd.

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