Influence of interface–dipole interactions on the efficiency of fluorescence light collection near surfaces

Abstract

Recently Török and colleagues published in a series of papers a general wave-optical approach to calculating the light-collection efficiency function (CEF) for confocal imaging of dipole emitters [J. Mod. Opt. 45, 1681 (1998); J. Microsc. 194, 127 (1999); Opt. Lett. 25, 1463 (2000)]. In their theory they did not address the possibility that the lifetimes of fluorescing molecules can change significantly near interfaces, which has a direct effect on the CEF. The research of Török and colleagues is extended here to include this effect, which may become important for imaging near surfaces.

© 2003 Optical Society of America

Theoretical study of detection of a dipole emitter through an objective with high numerical aperture

Jörg Enderlein
Opt. Lett. 25(9) 634-636 (2000)

Propagation of electromagnetic dipole waves through dielectric interfaces

P. Török
Opt. Lett. 25(19) 1463-1465 (2000)

Light emission by magnetic and electric dipoles close to a plane dielectric interface. III. Radiation patterns of dipoles with arbitrary orientation

W. Lukosz
J. Opt. Soc. Am. 69(11) 1495-1503 (1979)

Vector-based plane-wave spectrum method for the propagation of cylindrical electromagnetic fields

Shouyuan Shi and Dennis W. Prather
Opt. Lett. 24(21) 1445-1447 (1999)

Properties of a localized Mathieu pulse

C. A. Dartora and H. E. Hernández-Figueroa
J. Opt. Soc. Am. A 21(4) 662-667 (2004)