Abstract

We report the results of a study carried out to investigate the effect of blood absorption on polarized and unpolarized fluorescence from resected tissue samples and tissue phantoms. The signatures of blood absorption were found to be significantly smaller in polarized fluorescence than in unpolarized fluorescence spectra. The reduced effect of blood absorption on polarized fluorescence also leads to reduced site-to-site variability in polarized fluorescence intensity and line shape compared with unpolarized fluorescence.

© 2002 Optical Society of America

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References

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    [CrossRef]
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    [CrossRef]

2002 (1)

N. Ghosh, S. K. Majumder, and P. K. Gupta, Phys. Rev. E 65, 266081 (2002).

2001 (2)

2000 (1)

N. Ramanujam, Neoplasia 2(1), 1 (2000).

1998 (1)

G. A. Wagnieres, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998).
[CrossRef]

1994 (1)

D. Bicont, C. Brosseu, S. Martinez, and J. M. Schmitt, Phys. Rev. E 49, 1767 (1994).
[CrossRef]

1989 (1)

Alfano, R. R.

Bicont, D.

D. Bicont, C. Brosseu, S. Martinez, and J. M. Schmitt, Phys. Rev. E 49, 1767 (1994).
[CrossRef]

Bohren, C. F.

C. F. Bohren and D. R. Hoffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).

Brosseu, C.

D. Bicont, C. Brosseu, S. Martinez, and J. M. Schmitt, Phys. Rev. E 49, 1767 (1994).
[CrossRef]

Chen, J.

Ghosh, N.

Gupta, P. K.

Hoffman, D. R.

C. F. Bohren and D. R. Hoffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).

Lackowicz, J.

J. Lackowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983).
[CrossRef]

Liu, C. H.

Majumder, S. K.

Martinez, S.

D. Bicont, C. Brosseu, S. Martinez, and J. M. Schmitt, Phys. Rev. E 49, 1767 (1994).
[CrossRef]

Mohanty, S. K.

Pradhan, A.

Ramanujam, N.

N. Ramanujam, Neoplasia 2(1), 1 (2000).

Schmitt, J. M.

D. Bicont, C. Brosseu, S. Martinez, and J. M. Schmitt, Phys. Rev. E 49, 1767 (1994).
[CrossRef]

Sha, W.

Star, W. M.

G. A. Wagnieres, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998).
[CrossRef]

Tang, G. C.

Wagnieres, G. A.

G. A. Wagnieres, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998).
[CrossRef]

Wahl, S. J.

Wilson, B. C.

G. A. Wagnieres, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998).
[CrossRef]

Appl. Opt. (3)

Neoplasia (1)

N. Ramanujam, Neoplasia 2(1), 1 (2000).

Photochem. Photobiol. (1)

G. A. Wagnieres, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998).
[CrossRef]

Phys. Rev. E (2)

D. Bicont, C. Brosseu, S. Martinez, and J. M. Schmitt, Phys. Rev. E 49, 1767 (1994).
[CrossRef]

N. Ghosh, S. K. Majumder, and P. K. Gupta, Phys. Rev. E 65, 266081 (2002).

Other (2)

C. F. Bohren and D. R. Hoffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).

J. Lackowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

340-nm excited (a) unpolarized and (b) polarized fluorescence spectra recorded from human rectal tissue before (solid curves) and after (dashed curves) extraction of blood from the sample. Insets, the fluorescence spectra normalized with respect to integrated fluorescence intensity.

Fig. 2
Fig. 2

(a) Unpolarized and (b) polarized fluorescence spectra recorded from different tissue phantoms with 460-nm excitation. For all the samples μs was 3.38 mm-1 at 545 nm. Solid curve, sample with μa=0 mm-1; dashed curve, μa=0.014 mm-1; pluses, μa=0.028 mm-1; open circles, μa=0.056 mm-1; stars, μa=3.90 mm-1. The insets show the fluorescence spectra (normalized with respect to integrated fluorescence intensity) for three samples with μa values of 0, 0.056, and 3.90 mm-1.

Fig. 3
Fig. 3

340-nm excited (a) unpolarized and (b) polarized fluorescence spectra recorded from five sites of a tissue sample. Insets, the fluorescence spectra from the same sites normalized with respect to integrated fluorescence intensity.

Equations (1)

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Ipolλ=Iλ-G×Iλ,

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