Abstract
We report polarization coherent anti-Stokes Raman scattering (P-CARS) microscopy
that allows vibrational imaging with high sensitivity and spectral selectivity.
The nonresonant background signals from both Raman scatterers and the solvent
are efficiently suppressed in P-CARS microscopy. We demonstrate P-CARS imaging
of unstained cells based on the contrast of the protein amide I band.
© 2001 Optical Society of America
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