Abstract

We report a novel whole-field three-dimensional fluorescence lifetime imaging microscope that incoporates multispectral imaging to provide five-dimensional (5-D) fluorescence microscopy. This instrument, which can acquire a 5-D data set in less than a minute, is based on potentially compact and inexpensive diode-pumped solid-state laser technology. We demonstrate that spectral discrimination as well as optical sectioning minimize artifacts in lifetime determination and illustrate how spectral discrimination improves the lifetime contrast of biological tissue.

© 2001 Optical Society of America

Full Article  |  PDF Article
Related Articles
Whole-field optically sectioned fluorescence lifetime imaging

M. J. Cole, J. Siegel, S. E. D. Webb, R. Jones, K. Dowling, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. A. Neil, R. Juškaitis, and T. Wilson
Opt. Lett. 25(18) 1361-1363 (2000)

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

Jan Siegel, Daniel S. Elson, Stephen E. D. Webb, K. C. Benny Lee, Alexis Vlandas, Giovanni L. Gambaruto, Sandrine Lévêque-Fort, M. John Lever, Paul J. Tadrous, Gordon W. H. Stamp, Andrew L. Wallace, Ann Sandison, Tim F. Watson, Fernando Alvarez, and Paul M. W. French
Appl. Opt. 42(16) 2995-3004 (2003)

Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

Dylan M. Owen, Egidijus Auksorius, Hugh B. Manning, Clifford B. Talbot, Pieter A. A. de Beule, Christopher Dunsby, Mark A. A. Neil, and Paul M. W. French
Opt. Lett. 32(23) 3408-3410 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription