Abstract

We describe a new design for a microscope-based static light-scattering instrument that provides simultaneous high-resolution images and static light-scattering data. By correlating real space images with scattering patterns, we can interpret measurements from heterogeneous samples, which we illustrate by using biological tissue.

© 2001 Optical Society of America

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  1. I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
    [CrossRef] [PubMed]
  2. T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
    [CrossRef]
  3. D. E. Burger, J. H. Jett, and P. F. Mullaney, Proc. SPIE 190, 467 (1979).
    [CrossRef]
  4. P. S. Blank, R. B. Tishler, and F. D. Carlson, Appl. Opt. 26, 351 (1987).
    [CrossRef] [PubMed]
  5. P. D. Kaplan, V. Trappe, and D. A. Weitz, Appl. Opt. 38, 4151 (1999).
    [CrossRef]
  6. S. Inoue and K. R. Spring, Video Microscopy: The Fundamentals (Plenum, New York, 1997).
    [CrossRef]
  7. H. C. van de Hulst, Light Scattering by Small Particles (Dover, New York, 1981).
  8. A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
    [CrossRef]
  9. J. B. Fishkin, O. Coquoz, E. R. Anderson, M. Brenner, and B. J. Tromberg, Appl. Opt. 36, 10 (1997).
    [CrossRef] [PubMed]
  10. G. J. Tearney, M. E. Brezinski, and J. G. Fujimoto, Science 276, 2037 (1997).
    [CrossRef] [PubMed]
  11. J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
    [CrossRef]
  12. B. Beauvoit and B. Chance, Mol. Cell. Biochem. 184, 445 (1998).
    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  14. A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, Phys. Med. Biol. 43, 1285 (1998).
    [CrossRef] [PubMed]
  15. B. C. Wilson and G. Adam, Med. Phys. 10, 824 (1983).
    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]

2000 (1)

A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
[CrossRef]

1999 (1)

1998 (2)

B. Beauvoit and B. Chance, Mol. Cell. Biochem. 184, 445 (1998).
[CrossRef] [PubMed]

A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, Phys. Med. Biol. 43, 1285 (1998).
[CrossRef] [PubMed]

1997 (3)

1995 (2)

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

I. S. Saidi, S. L. Jacques, and F. K. Tittel, Appl. Opt. 34, 7410 (1995).
[CrossRef] [PubMed]

1993 (1)

T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
[CrossRef]

1987 (1)

1983 (2)

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

B. C. Wilson and G. Adam, Med. Phys. 10, 824 (1983).
[CrossRef] [PubMed]

1979 (1)

D. E. Burger, J. H. Jett, and P. F. Mullaney, Proc. SPIE 190, 467 (1979).
[CrossRef]

Adam, G.

B. C. Wilson and G. Adam, Med. Phys. 10, 824 (1983).
[CrossRef] [PubMed]

Alcouffe, R. E.

A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, Phys. Med. Biol. 43, 1285 (1998).
[CrossRef] [PubMed]

Anderson, E. R.

Barbour, R. L.

A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, Phys. Med. Biol. 43, 1285 (1998).
[CrossRef] [PubMed]

Beauvoit, B.

B. Beauvoit and B. Chance, Mol. Cell. Biochem. 184, 445 (1998).
[CrossRef] [PubMed]

Bigio, I. J.

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Blank, P. S.

Boyer, J.

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Brenner, M.

Brezinski, M. E.

G. J. Tearney, M. E. Brezinski, and J. G. Fujimoto, Science 276, 2037 (1997).
[CrossRef] [PubMed]

Burger, D. E.

D. E. Burger, J. H. Jett, and P. F. Mullaney, Proc. SPIE 190, 467 (1979).
[CrossRef]

Carlson, F. D.

Chance, B.

B. Beauvoit and B. Chance, Mol. Cell. Biochem. 184, 445 (1998).
[CrossRef] [PubMed]

Conn, R. L.

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Cope, M.

Coquoz, O.

Delpy, D. T.

Fishkin, J. B.

Fujimoto, J. G.

G. J. Tearney, M. E. Brezinski, and J. G. Fujimoto, Science 276, 2037 (1997).
[CrossRef] [PubMed]

Hielscher, A. H.

A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, Phys. Med. Biol. 43, 1285 (1998).
[CrossRef] [PubMed]

Imanishi, Y.

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

Inoue, S.

S. Inoue and K. R. Spring, Video Microscopy: The Fundamentals (Plenum, New York, 1997).
[CrossRef]

Ishiwata, S.

T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
[CrossRef]

Jacques, S. L.

Jett, J. H.

D. E. Burger, J. H. Jett, and P. F. Mullaney, Proc. SPIE 190, 467 (1979).
[CrossRef]

Johnson, T.

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Kaplan, P. D.

A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
[CrossRef]

P. D. Kaplan, V. Trappe, and D. A. Weitz, Appl. Opt. 38, 4151 (1999).
[CrossRef]

Matcher, S. J.

Mourant, J. R.

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Mullaney, P. F.

D. E. Burger, J. H. Jett, and P. F. Mullaney, Proc. SPIE 190, 467 (1979).
[CrossRef]

Nishio, I.

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

Nishizaki, T.

T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
[CrossRef]

Ohnishi, S. T.

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

Popp, A. K.

A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
[CrossRef]

Saidi, I. S.

Shimada, T.

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Spring, K. R.

S. Inoue and K. R. Spring, Video Microscopy: The Fundamentals (Plenum, New York, 1997).
[CrossRef]

Sun, S.-T.

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

Tanaka, T.

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

Tanaka, Y.

T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
[CrossRef]

Tearney, G. J.

G. J. Tearney, M. E. Brezinski, and J. G. Fujimoto, Science 276, 2037 (1997).
[CrossRef] [PubMed]

Tishler, R. B.

Tittel, F. K.

Trappe, V.

Tromberg, B. J.

Valentine, M. T.

A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
[CrossRef]

van de Hulst, H. C.

H. C. van de Hulst, Light Scattering by Small Particles (Dover, New York, 1981).

Weitz, D. A.

A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
[CrossRef]

P. D. Kaplan, V. Trappe, and D. A. Weitz, Appl. Opt. 38, 4151 (1999).
[CrossRef]

Wilson, B. C.

B. C. Wilson and G. Adam, Med. Phys. 10, 824 (1983).
[CrossRef] [PubMed]

Yagi, T.

T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
[CrossRef]

Appl. Opt. (5)

Lasers Surg. Med. (1)

J. R. Mourant, I. J. Bigio, J. Boyer, R. L. Conn, T. Johnson, and T. Shimada, Lasers Surg. Med. 17, 350 (1995).
[CrossRef]

Med. Phys. (1)

B. C. Wilson and G. Adam, Med. Phys. 10, 824 (1983).
[CrossRef] [PubMed]

Mol. Cell. Biochem. (1)

B. Beauvoit and B. Chance, Mol. Cell. Biochem. 184, 445 (1998).
[CrossRef] [PubMed]

Nature (1)

T. Nishizaki, T. Yagi, Y. Tanaka, and S. Ishiwata, Nature 361, 269 (1993).
[CrossRef]

Phys. Med. Biol. (1)

A. H. Hielscher, R. E. Alcouffe, and R. L. Barbour, Phys. Med. Biol. 43, 1285 (1998).
[CrossRef] [PubMed]

Proc. SPIE (2)

D. E. Burger, J. H. Jett, and P. F. Mullaney, Proc. SPIE 190, 467 (1979).
[CrossRef]

A. K. Popp, M. T. Valentine, P. D. Kaplan, and D. A. Weitz, Proc. SPIE 3917, 22 (2000).
[CrossRef]

Science (2)

I. Nishio, T. Tanaka, S.-T. Sun, Y. Imanishi, and S. T. Ohnishi, Science 220, 1173 (1983).
[CrossRef] [PubMed]

G. J. Tearney, M. E. Brezinski, and J. G. Fujimoto, Science 276, 2037 (1997).
[CrossRef] [PubMed]

Other (2)

S. Inoue and K. R. Spring, Video Microscopy: The Fundamentals (Plenum, New York, 1997).
[CrossRef]

H. C. van de Hulst, Light Scattering by Small Particles (Dover, New York, 1981).

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Figures (3)

Fig. 1
Fig. 1

Schematic of the optical arrangement of the SLS microscope.

Fig. 2
Fig. 2

Iq measured from a dilute suspension of 2.0μm spheres in a 1:1 mixture of H2O:D2O at ϕ=10-3. Solid curve, form factor predicted from Mie theory with no fitting parameters.

Fig. 3
Fig. 3

A, B, Real-space and scattering-space images from a monolayer of 2.0μm particles: A, a region with one dominant crystal orientation, resulting in pronounced Bragg peaks; B, regions with polycrystalline domains, resulting in a Bragg ring and indicating the average nearest-neighbor distance, 2.00±0.04 μm. C, D, Real-space and scattering-space images from porcine skin tissue: C, The real-space image shows a region devoid of any large heterogeneities; the resultant scattering pattern is isotropic. D, A region from the same slice in the vicinity of a hair, resulting in an anisotropic scattering pattern.

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