Abstract

We have developed a new coherent anti-Stokes Raman scattering (CARS) microscopy system with a collinear configuration for use in the fingerprint region. The system consists of a picosecond laser system and a transmission-type laser scanning microscope without a pinhole in front of the detector. The observable Raman-shift region is 9001750 cm-1, the spectral resolution is 30 cm-1, and the spatial resolution is smaller than 1 µm in the lateral direction and 3.2 µm in the depth direction, with objectives with a numerical aperture of 0.65. CARS spectra and images of polystyrene beads are demonstrated, and CARS imaging of a viable yeast cell is attempted.

© 2000 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging

Takeo Minamikawa, Mamoru Hashimoto, Katsumasa Fujita, Satoshi Kawata, and Tsutomu Araki
Opt. Express 17(12) 9526-9536 (2009)

Quantitative chemical imaging with background-free multiplex coherent anti-Stokes Raman scattering by dual-soliton Stokes pulses

Kun Chen, Tao Wu, Haoyun Wei, Tian Zhou, and Yan Li
Biomed. Opt. Express 7(10) 3927-3939 (2016)

Coherent anti-Stokes Raman scattering microscopy using a single-pass picosecond supercontinuum-seeded optical parametric amplifier

Chao-Yu Chung, Yen-Yin Lin, Kuo-Yu Wu, Wan-Yu Tai, Shi-Wei Chu, Yao-Chang Lee, Yeukuang Hwu, and Yin-Yu Lee
Opt. Express 18(6) 6116-6122 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription