Abstract

We have developed a new coherent anti-Stokes Raman scattering (CARS) microscopy system with a collinear configuration for use in the fingerprint region. The system consists of a picosecond laser system and a transmission-type laser scanning microscope without a pinhole in front of the detector. The observable Raman-shift region is 900–1750 cm-1, the spectral resolution is 30 cm-1, and the spatial resolution is smaller than 1 µm in the lateral direction and 3.2 µm in the depth direction, with objectives with a numerical aperture of 0.65. CARS spectra and images of polystyrene beads are demonstrated, and CARS imaging of a viable yeast cell is attempted.

© 2000 Optical Society of America

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  1. W. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Eng. 24, 352 (1985).
  2. M. D. Duncan, Opt. Commun. 50, 307 (1984).
    [CrossRef]
  3. M. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Lett. 7, 350 (1982).
    [CrossRef] [PubMed]
  4. A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
    [CrossRef]
  5. G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
    [CrossRef] [PubMed]
  6. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
    [CrossRef] [PubMed]
  7. M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
    [CrossRef]
  8. M. D. Levenson and S. Kano, Introduction to Nonlinear Laser Spectroscopy (Academic, Orlando, Fla., 1988).
  9. Y. R. Shen, Principles of Nonlinear Optics (Wiley, New York, 1984).

2000 (1)

M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
[CrossRef]

1999 (1)

A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
[CrossRef]

1990 (2)

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

1985 (1)

W. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Eng. 24, 352 (1985).

1984 (1)

M. D. Duncan, Opt. Commun. 50, 307 (1984).
[CrossRef]

1982 (1)

Arndt-Jovin, D. J.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Brakenhoff, G. J.

M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
[CrossRef]

de Lange, C. A.

M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
[CrossRef]

de Mul, F. F. M.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Duncan, M. D.

Duncan, W. D.

W. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Eng. 24, 352 (1985).

Greve, J.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Holtom, G. R.

A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
[CrossRef]

Jovin, T. M.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Kano, S.

M. D. Levenson and S. Kano, Introduction to Nonlinear Laser Spectroscopy (Academic, Orlando, Fla., 1988).

Levenson, M. D.

M. D. Levenson and S. Kano, Introduction to Nonlinear Laser Spectroscopy (Academic, Orlando, Fla., 1988).

Manuccia, T. J.

W. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Eng. 24, 352 (1985).

M. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Lett. 7, 350 (1982).
[CrossRef] [PubMed]

Muller, M.

M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
[CrossRef]

Otto, C.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Puppels, G. J.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Reintjes, J.

W. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Eng. 24, 352 (1985).

M. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Lett. 7, 350 (1982).
[CrossRef] [PubMed]

Robert-Nicoud, M.

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Shen, Y. R.

Y. R. Shen, Principles of Nonlinear Optics (Wiley, New York, 1984).

Squier, J.

M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
[CrossRef]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Webb, W. W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Xie, X. S.

A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
[CrossRef]

Zumbusch, A.

A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
[CrossRef]

J. Microsc. (Oxford) (1)

M. Muller, J. Squier, C. A. de Lange, and G. J. Brakenhoff, J. Microsc. (Oxford) 197, 150 (2000).
[CrossRef]

Nature (1)

G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, Nature 347, 301 (1990).
[CrossRef] [PubMed]

Opt. Commun. (1)

M. D. Duncan, Opt. Commun. 50, 307 (1984).
[CrossRef]

Opt. Eng. (1)

W. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Eng. 24, 352 (1985).

Opt. Lett. (1)

Phys. Rev. Lett. (1)

A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).
[CrossRef]

Science (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Other (2)

M. D. Levenson and S. Kano, Introduction to Nonlinear Laser Spectroscopy (Academic, Orlando, Fla., 1988).

Y. R. Shen, Principles of Nonlinear Optics (Wiley, New York, 1984).

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