Abstract

Ultrahigh-resolution optical coherence tomography (OCT) by use of state of the art broad-bandwidth femtosecond laser technology is demonstrated and applied to in vivo subcellular imaging. Imaging is performed with a Kerr-lens mode-locked Ti:sapphire laser with double-chirped mirrors that emits sub-two-cycle pulses with bandwidths of up to 350 nm, centered at 800 nm. Longitudinal resolutions of 1 µm and transverse resolution of 3 µm, with a 110-dB dynamic range, are achieved in biological tissue. To overcome depth-of-field limitations we perform zone focusing and image fusion to construct a tomogram with high transverse resolution throughout the image depth. To our knowledge this is the highest longitudinal resolution demonstrated to date for in vivo OCT imaging.

© 1999 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Contrast limits of coherence-gated imaging in scattering media

Yingtian Pan, Reginald Birngruber, and Ralf Engelhardt
Appl. Opt. 36(13) 2979-2983 (1997)

Patterned femtosecond-laser ablation of Xenopus laevis melanocytes for studies of cell migration, wound repair, and developmental processes

Jessica P. Mondia, Dany S. Adams, Ryan D. Orendorff, Michael Levin, and Fiorenzo G. Omenetto
Biomed. Opt. Express 2(8) 2383-2391 (2011)

High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe

Aaron D. Aguirre, Juergen Sawinski, Shu-Wei Huang, Chao Zhou, Winfried Denk, and James G. Fujimoto
Opt. Express 18(5) 4222-4239 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription