Abstract

We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited molecules from the outer part of the focus through stimulated emission. This results in a subdiffraction-sized effective point-spread function. For a 1.4 aperture and a 388-nm excitation wavelength spatial resolution is increased from 150±8 nm to 106±8 nm with a single offset beam. Superior lateral resolution is demonstrated by separation of adjacent Pyridine 2 nanocrystals that are otherwise indiscernible.

© 1999 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Two-photon excitation and stimulated emission depletion by a single wavelength

Teodora Scheul, Ciro D’Amico, Irène Wang, and Jean-Claude Vial
Opt. Express 19(19) 18036-18048 (2011)

Time-gating improves the spatial resolution of STED microscopy

Jeffrey R. Moffitt, Christian Osseforth, and Jens Michaelis
Opt. Express 19(5) 4242-4254 (2011)

Analytical description of STED microscopy performance

Marcel Leutenegger, Christian Eggeling, and Stefan W. Hell
Opt. Express 18(25) 26417-26429 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription