Abstract

The D85N genetic variant of bacteriorhodopsin (BR) displays a nearly permanent lifetime of the photochromic P(490) state. We present pump–probe measurements that demonstrate this behavior. However, diffraction efficiency measurements made from holograms recorded in a hydrated D85N film show markedly different decay behavior, suggesting that a molecular diffusion process is occurring in the film. Holograms recorded with different grating frequencies display correspondingly different decay times, thus supporting this hypothesis. A thin D85N film was fabricated that was chemically cross linked, resulting in the elimination of diffusion of BR molecules within the polymer matrix. This film exhibits a grating lifetime of the order of weeks or more, thus permitting the long-term holographic storage of information.

© 1998 Optical Society of America

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References

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  1. J. D. Downie and D. T. Smithey, Appl. Opt. 35, 5780 (1996).
    [CrossRef] [PubMed]
  2. A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
    [CrossRef] [PubMed]
  3. G. Váró and J. K. Lanyi, Biophys. J. 56, 1143 (1989).
    [CrossRef]

1996 (1)

1993 (1)

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

1989 (1)

G. Váró and J. K. Lanyi, Biophys. J. 56, 1143 (1989).
[CrossRef]

Brauchle, C.

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

Downie, J. D.

Hampp, N.

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

Lanyi, J. K.

G. Váró and J. K. Lanyi, Biophys. J. 56, 1143 (1989).
[CrossRef]

Oesterhelt, D.

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

Popp, A.

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

Smithey, D. T.

Váró, G.

G. Váró and J. K. Lanyi, Biophys. J. 56, 1143 (1989).
[CrossRef]

Wolperdinger, M.

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

Appl. Opt. (1)

Biophys. J. (2)

A. Popp, M. Wolperdinger, N. Hampp, C. Brauchle, and D. Oesterhelt, Biophys. J. 65, 1449 (1993).
[CrossRef] [PubMed]

G. Váró and J. K. Lanyi, Biophys. J. 56, 1143 (1989).
[CrossRef]

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Figures (4)

Fig. 1
Fig. 1

Measured absorption spectra of B state and bleached P(490) state molecules in D85N film.

Fig. 2
Fig. 2

Transmitted power of a 633-nm probe beam through D85N film after the pump beam is turned off.

Fig. 3
Fig. 3

Diffraction efficiency of a 633-nm readout beam as a function of time after the recording beams are turned off.

Fig. 4
Fig. 4

Diffraction efficiency of a cross-linked D85N film at 633-nm after the recording beams are turned off.

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