We applied phase-shifting digital holography to microscopy by deriving
the complex amplitude of light scattered from microscopic three-dimensional
objects through a microscope objective by video camera recording, phase-shifting
analysis, and computer reconstruction. This method requires no mechanical
movement and provides a flexible display and quantitative evaluation of the
reconstructed images. A theory of image formation and experimental verification
with specimens are described.
© 1998 Optical Society of America
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