Abstract

We describe a novel whole-field fluorescence lifetime imaging system, based on a time-gated image intensifier and a solid-state laser oscillator–amplifier, that images lifetime differences of less than 10  ps. This system was successfully applied to discrimination between biological tissue constituents.

© 1998 Optical Society of America

Full Article  |  PDF Article
Related Articles
Whole-field five-dimensional fluorescence microscopy combining lifetime and spectral resolution with optical sectioning

J. Siegel, D. S. Elson, S. E. D. Webb, D. Parsons-Karavassilis, S. Lévêque-Fort, M. J. Cole, M. J. Lever, P. M. W. French, M. A. A. Neil, R. Juŝkaitis, L. O. Sucharov, and T. Wilson
Opt. Lett. 26(17) 1338-1340 (2001)

Fluorescence lifetime system for microscopy and multiwell plate imaging with a blue picosecond diode laser

D. S. Elson, J. Siegel, S. E. D. Webb, S. Lévêque-Fort, M. J. Lever, P. M. W. French, K. Lauritsen, M. Wahl, and R. Erdmann
Opt. Lett. 27(16) 1409-1411 (2002)

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

Jan Siegel, Daniel S. Elson, Stephen E. D. Webb, K. C. Benny Lee, Alexis Vlandas, Giovanni L. Gambaruto, Sandrine Lévêque-Fort, M. John Lever, Paul J. Tadrous, Gordon W. H. Stamp, Andrew L. Wallace, Ann Sandison, Tim F. Watson, Fernando Alvarez, and Paul M. W. French
Appl. Opt. 42(16) 2995-3004 (2003)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription