Abstract

Video-enhanced optical fluorescence microscopy has been used to determine the direction of the transition dipole moment of individual molecules. Fast electro-optical switching of the polarization of the excitation allowed us to detect the linear dichroism of single fluorophores in solid phospholipid membranes. Quantitative analysis of the fluorescence signal showed that rotational mobility is absent in solid biomembranes.

© 1997 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. E. Betzig and R. J. Chichester, Science 262, 1422 (1993).
    [CrossRef] [PubMed]
  2. D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
    [CrossRef]
  3. D. Sabbert, S. Engelbrecht, and W. Junge, Nature (London) 380, 623 (1996).
    [CrossRef]
  4. T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
    [CrossRef] [PubMed]
  5. E. Sackmann, in Structure and Dynamics of Membranes, A. J. Hoff, ed. (Elsevier, Amsterdam, 1995), p. 1.
  6. M. Velez and D. Axelrod, Biophys. J. 53, 575 (1988).
    [CrossRef] [PubMed]
  7. R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
    [CrossRef]
  8. T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
    [CrossRef]
  9. X. S. Xie and R. C. Dunn, Science 265, 361 (1994).
    [CrossRef] [PubMed]
  10. H. Frauenfelder, Science 254, 1598 (1991).
    [CrossRef] [PubMed]
  11. Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
    [CrossRef]
  12. Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
    [CrossRef]
  13. L. K. Tamm and H. M. McConnel, Biophys. J. 47, 105 (1985).
    [CrossRef] [PubMed]
  14. M. Lieberherr, Ch. Fattinger, and W. Lukosz, Surf. Sci. 189, 664 (1987).

1996 (6)

D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
[CrossRef]

D. Sabbert, S. Engelbrecht, and W. Junge, Nature (London) 380, 623 (1996).
[CrossRef]

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

1995 (1)

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

1994 (1)

X. S. Xie and R. C. Dunn, Science 265, 361 (1994).
[CrossRef] [PubMed]

1993 (1)

E. Betzig and R. J. Chichester, Science 262, 1422 (1993).
[CrossRef] [PubMed]

1991 (1)

H. Frauenfelder, Science 254, 1598 (1991).
[CrossRef] [PubMed]

1988 (1)

M. Velez and D. Axelrod, Biophys. J. 53, 575 (1988).
[CrossRef] [PubMed]

1987 (1)

M. Lieberherr, Ch. Fattinger, and W. Lukosz, Surf. Sci. 189, 664 (1987).

1985 (1)

L. K. Tamm and H. M. McConnel, Biophys. J. 47, 105 (1985).
[CrossRef] [PubMed]

Axelrod, D.

M. Velez and D. Axelrod, Biophys. J. 53, 575 (1988).
[CrossRef] [PubMed]

Barbara, P. F.

D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
[CrossRef]

Baumgartner, W.

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

Betzig, E.

E. Betzig and R. J. Chichester, Science 262, 1422 (1993).
[CrossRef] [PubMed]

Chemla, D. S.

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

Chichester, R. J.

E. Betzig and R. J. Chichester, Science 262, 1422 (1993).
[CrossRef] [PubMed]

Dunn, R. C.

X. S. Xie and R. C. Dunn, Science 265, 361 (1994).
[CrossRef] [PubMed]

Enderle, Th.

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

Engelbrecht, S.

D. Sabbert, S. Engelbrecht, and W. Junge, Nature (London) 380, 623 (1996).
[CrossRef]

Fattinger, Ch.

M. Lieberherr, Ch. Fattinger, and W. Lukosz, Surf. Sci. 189, 664 (1987).

Frauenfelder, H.

H. Frauenfelder, Science 254, 1598 (1991).
[CrossRef] [PubMed]

Funatsu, T.

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

Gruber, H. J.

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

Ha, T.

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

Harada, Y.

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

Higgins, D. A.

D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
[CrossRef]

Junge, W.

D. Sabbert, S. Engelbrecht, and W. Junge, Nature (London) 380, 623 (1996).
[CrossRef]

Kerimo, J.

D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
[CrossRef]

Lieberherr, M.

M. Lieberherr, Ch. Fattinger, and W. Lukosz, Surf. Sci. 189, 664 (1987).

Lukosz, W.

M. Lieberherr, Ch. Fattinger, and W. Lukosz, Surf. Sci. 189, 664 (1987).

McConnel, H. M.

L. K. Tamm and H. M. McConnel, Biophys. J. 47, 105 (1985).
[CrossRef] [PubMed]

Ogletree, D. F.

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

Pierce, D. W.

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

Romberg, L.

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

Sabbert, D.

D. Sabbert, S. Engelbrecht, and W. Junge, Nature (London) 380, 623 (1996).
[CrossRef]

Sackmann, E.

E. Sackmann, in Structure and Dynamics of Membranes, A. J. Hoff, ed. (Elsevier, Amsterdam, 1995), p. 1.

Schindler, H.

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

Schmidt, Th.

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

Schütz, G. J.

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

Selvin, P. R.

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

Tamm, L. K.

L. K. Tamm and H. M. McConnel, Biophys. J. 47, 105 (1985).
[CrossRef] [PubMed]

Vale, R. D.

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

van den Bouten, D. A.

D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
[CrossRef]

Velez, M.

M. Velez and D. Axelrod, Biophys. J. 53, 575 (1988).
[CrossRef] [PubMed]

Weiss, S.

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

Xie, X. S.

X. S. Xie and R. C. Dunn, Science 265, 361 (1994).
[CrossRef] [PubMed]

Yanagida, T.

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

Biophys. J. (2)

M. Velez and D. Axelrod, Biophys. J. 53, 575 (1988).
[CrossRef] [PubMed]

L. K. Tamm and H. M. McConnel, Biophys. J. 47, 105 (1985).
[CrossRef] [PubMed]

J. Phys. Chem. (2)

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17662 (1995).
[CrossRef]

D. A. Higgins, D. A. van den Bouten, J. Kerimo, and P. F. Barbara, J. Phys. Chem. 100, 13794 (1996).
[CrossRef]

Nature (London) (2)

D. Sabbert, S. Engelbrecht, and W. Junge, Nature (London) 380, 623 (1996).
[CrossRef]

R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida, Nature (London) 380, 451 (1996).
[CrossRef]

Phys. Rev. Lett. (1)

T. Ha, Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci. USA (2)

T. Ha, Th. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. USA 93, 6264 (1996).
[CrossRef]

Th. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. USA 93, 2926 (1996).
[CrossRef]

Science (3)

E. Betzig and R. J. Chichester, Science 262, 1422 (1993).
[CrossRef] [PubMed]

X. S. Xie and R. C. Dunn, Science 265, 361 (1994).
[CrossRef] [PubMed]

H. Frauenfelder, Science 254, 1598 (1991).
[CrossRef] [PubMed]

Surf. Sci. (1)

M. Lieberherr, Ch. Fattinger, and W. Lukosz, Surf. Sci. 189, 664 (1987).

Other (1)

E. Sackmann, in Structure and Dynamics of Membranes, A. J. Hoff, ed. (Elsevier, Amsterdam, 1995), p. 1.

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (1)

Fig. 1
Fig. 1

a, Fluorescence images of a 5.4 µm×5.9 µm area of a solid phospholipid membrane. The images were taken with a repetition time of 15  ms while they were illuminated for 5  ms, and scaled between 0 (white) and 40 (black) photon counts. b, Polarization of the excitation laser beam was switched between 0 and π/2 as indicated by the square wave. c, d, Photon counts for both molecules observed in a.

Metrics