Abstract

We measure the point-spread function in the two main configurations of 4Pi confocal microscopy as well as in the traditional confocal arrangement and derive the optical transfer functions from the experimental data. The optical transfer functions are in good agreement with their theoretical counterparts. We find a 3.5- to 5-fold increased axial bandwidth of the 4Pi confocal microscope and hence confirm the enhanced spatial-frequency content of 4Pi images.

© 1997 Optical Society of America

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