Abstract

A novel noninvasive second-harmonic-generation tomographic method of mapping the structure of animal tissues by use of 100-fs laser pulses at 625  nm is described. Subsurface structures were measured with this approach, which is potentially a symmetry-sensitive tool for optical histological reconstruction.

© 1997 Optical Society of America

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References

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
    [CrossRef] [PubMed]
  2. D. W. Piston, B. R. Masters, and W. W. Webb, J. Microsc. 178, 20 (1994), and references therein.
    [CrossRef]
  3. I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986).
    [CrossRef] [PubMed]
  4. D. Benaron and D. Stevenson, Science 259, 1463 (1993).
    [CrossRef] [PubMed]
  5. L. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, Science 253, 769 (1991).
    [CrossRef] [PubMed]
  6. W. Denk, J. Biomed. Opt. 1, 296 (1996).
    [CrossRef] [PubMed]
  7. S. Fine and W. P. Hansen, Appl. Opt. 10, 2350 (1971).
    [CrossRef] [PubMed]
  8. Y. Guo, P. P. Ho, A. Tirkisliunas, F. Liu, and R. R. Alfano, Appl. Opt. 35, 6810 (1996).
    [CrossRef] [PubMed]
  9. T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
    [CrossRef]

1996 (2)

1994 (1)

D. W. Piston, B. R. Masters, and W. W. Webb, J. Microsc. 178, 20 (1994), and references therein.
[CrossRef]

1993 (1)

D. Benaron and D. Stevenson, Science 259, 1463 (1993).
[CrossRef] [PubMed]

1991 (2)

L. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, Science 253, 769 (1991).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

1986 (1)

I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986).
[CrossRef] [PubMed]

1982 (1)

T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
[CrossRef]

1971 (1)

Alfano, R. R.

Benaron, D.

D. Benaron and D. Stevenson, Science 259, 1463 (1993).
[CrossRef] [PubMed]

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Chen, C. K.

T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
[CrossRef]

Denk, W.

W. Denk, J. Biomed. Opt. 1, 296 (1996).
[CrossRef] [PubMed]

Deutsch, M.

I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986).
[CrossRef] [PubMed]

Fine, S.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Freund, I.

I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986).
[CrossRef] [PubMed]

Fujimoto, J. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Guo, Y.

Hansen, W. P.

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Heinz, T. F.

T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
[CrossRef]

Ho, P. P.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Liu, C.

L. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, Science 253, 769 (1991).
[CrossRef] [PubMed]

Liu, F.

Masters, B. R.

D. W. Piston, B. R. Masters, and W. W. Webb, J. Microsc. 178, 20 (1994), and references therein.
[CrossRef]

Piston, D. W.

D. W. Piston, B. R. Masters, and W. W. Webb, J. Microsc. 178, 20 (1994), and references therein.
[CrossRef]

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Richard, D.

T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
[CrossRef]

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Shen, Y. R.

T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
[CrossRef]

Sprecher, A.

I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986).
[CrossRef] [PubMed]

Stevenson, D.

D. Benaron and D. Stevenson, Science 259, 1463 (1993).
[CrossRef] [PubMed]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Tirkisliunas, A.

Wang, L.

L. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, Science 253, 769 (1991).
[CrossRef] [PubMed]

Webb, W. W.

D. W. Piston, B. R. Masters, and W. W. Webb, J. Microsc. 178, 20 (1994), and references therein.
[CrossRef]

Zhang, G.

L. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, Science 253, 769 (1991).
[CrossRef] [PubMed]

Appl. Opt. (2)

Biophys. J. (1)

I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986).
[CrossRef] [PubMed]

J. Biomed. Opt. (1)

W. Denk, J. Biomed. Opt. 1, 296 (1996).
[CrossRef] [PubMed]

J. Microsc. (1)

D. W. Piston, B. R. Masters, and W. W. Webb, J. Microsc. 178, 20 (1994), and references therein.
[CrossRef]

Phys. Rev. Lett. (1)

T. F. Heinz, C. K. Chen, D. Richard, and Y. R. Shen, Phys. Rev. Lett. 48, 478 (1982).
[CrossRef]

Science (3)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

D. Benaron and D. Stevenson, Science 259, 1463 (1993).
[CrossRef] [PubMed]

L. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, Science 253, 769 (1991).
[CrossRef] [PubMed]

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Figures (5)

Fig. 1
Fig. 1

Schematic of the experimental setup: MC, microscope objective; BS, beam splitter; F1, F2, long and narrow bandpass filters, respectively; PMT, photomultiplier tube.

Fig. 2
Fig. 2

(a) 2D SHG depth image of chicken skin tissue, log ISHG versus y, z. (b) Analog axially scanned SHG profiles in linear scale.

Fig. 3
Fig. 3

(a) 2D SHG depth image of a fascia membrane attached to chicken muscle tissue, log ISHG versus y, z. (b) Analog axially scanned SHG profiles in linear scale.

Fig. 4
Fig. 4

(a) 2D SHG depth image of chicken muscle tissue, log ISHG versus y, z. (b) Analog axially scanned SHG profiles in linear scale.

Fig. 5
Fig. 5

Analog z-scanned SHG depth profile of tryptophan powder.

Equations (1)

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I2ω16πω/nω2n2ωc2κS2ω2χeff22Iω2,

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