Abstract

We report the operation of an aerosol-fluorescence spectrum analyzer capable of selectively measuring the fluorescence spectra of single micrometer-sized aerosol particles as they flow through the instrument. As the particle first traverses a cw 488-nm probe laser beam, the total fluorescence and elastic scattering are measured with photomultipliers. When the photomultiplier output levels meet preset logic conditions, a UV laser (at 266 nm) is fired and the particle fluorescence spectrum is recorded. Fluorescence spectra of biological airborne particles are presented. The ability of the analyzer to capture the fluorescence spectrum of one type of particle while ignoring others, based on the particle characteristics, is also demonstrated.

© 1996 Optical Society of America

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  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983), Chap. 3, p. 341.
    [CrossRef]
  2. R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
    [CrossRef]
  3. S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 34, 7149 (1995).
    [CrossRef] [PubMed]
  4. P. Nachman, G. Chen, R. G. Pinnick, S. C. Hill, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 35, 1069 (1996).
    [CrossRef] [PubMed]
  5. M. W. Mayo, S. C. Hill, R. K. Chang, “Fluorescence of bacteria, pollens and naturally occurring airborne particles: excitation/emission spectra,” Appl. Spectrosc. (to be published).
  6. P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
    [CrossRef]
  7. F. W. J. Teale, G. Weber, Biochem. J. 65, 467 (1957).
  8. J. W. Longworth, in Excited States of Proteins and Nucleic Acids, R. F. Steiner, I. Weinryb, eds. (Plenum, New York, 1971), Chap. 6, p. 319.
    [CrossRef]

1996

1995

S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 34, 7149 (1995).
[CrossRef] [PubMed]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
[CrossRef]

P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
[CrossRef]

1957

F. W. J. Teale, G. Weber, Biochem. J. 65, 467 (1957).

Bruno, J. G.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
[CrossRef]

Carson, P. G.

P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
[CrossRef]

Chang, R. K.

P. Nachman, G. Chen, R. G. Pinnick, S. C. Hill, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 35, 1069 (1996).
[CrossRef] [PubMed]

S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 34, 7149 (1995).
[CrossRef] [PubMed]

M. W. Mayo, S. C. Hill, R. K. Chang, “Fluorescence of bacteria, pollens and naturally occurring airborne particles: excitation/emission spectra,” Appl. Spectrosc. (to be published).

Chen, G.

Fernandez, G. L.

Hill, S. C.

P. Nachman, G. Chen, R. G. Pinnick, S. C. Hill, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 35, 1069 (1996).
[CrossRef] [PubMed]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
[CrossRef]

S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 34, 7149 (1995).
[CrossRef] [PubMed]

M. W. Mayo, S. C. Hill, R. K. Chang, “Fluorescence of bacteria, pollens and naturally occurring airborne particles: excitation/emission spectra,” Appl. Spectrosc. (to be published).

Johnston, M. V.

P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
[CrossRef]

Lakowicz, J. R.

J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983), Chap. 3, p. 341.
[CrossRef]

Longworth, J. W.

J. W. Longworth, in Excited States of Proteins and Nucleic Acids, R. F. Steiner, I. Weinryb, eds. (Plenum, New York, 1971), Chap. 6, p. 319.
[CrossRef]

Mayo, M. W.

P. Nachman, G. Chen, R. G. Pinnick, S. C. Hill, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 35, 1069 (1996).
[CrossRef] [PubMed]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
[CrossRef]

S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 34, 7149 (1995).
[CrossRef] [PubMed]

M. W. Mayo, S. C. Hill, R. K. Chang, “Fluorescence of bacteria, pollens and naturally occurring airborne particles: excitation/emission spectra,” Appl. Spectrosc. (to be published).

Nachman, P.

Neubauer, K. R.

P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
[CrossRef]

Pendleton, J. D.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
[CrossRef]

Pinnick, R. G.

Teale, F. W. J.

F. W. J. Teale, G. Weber, Biochem. J. 65, 467 (1957).

Weber, G.

F. W. J. Teale, G. Weber, Biochem. J. 65, 467 (1957).

Wexler, A. S.

P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
[CrossRef]

Aerosol Sci. Technol.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
[CrossRef]

Appl. Opt.

Biochem. J.

F. W. J. Teale, G. Weber, Biochem. J. 65, 467 (1957).

J. Aerosol Sci.

P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995).
[CrossRef]

Other

J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983), Chap. 3, p. 341.
[CrossRef]

M. W. Mayo, S. C. Hill, R. K. Chang, “Fluorescence of bacteria, pollens and naturally occurring airborne particles: excitation/emission spectra,” Appl. Spectrosc. (to be published).

J. W. Longworth, in Excited States of Proteins and Nucleic Acids, R. F. Steiner, I. Weinryb, eds. (Plenum, New York, 1971), Chap. 6, p. 319.
[CrossRef]

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Figures (4)

Fig. 1
Fig. 1

(a) Schematic of the sample chamber. (b) Timing diagram for conditional firing of the UV laser.

Fig. 2
Fig. 2

To detect the fluorescence spectra of blue-dyed polystyrene spheres, 20 consecutive ICCD recordings are taken with the system in (a) the free-running mode and (b) the conditional-firing mode. The latter is conditioned on the 488-nm elastic scattering.

Fig. 3
Fig. 3

Accumulation of 100 conditional-firing UV-laser-excited fluorescence (Flu) spectra from (a) biological compounds and (b) bacterial particles. Conditional firing is based on the 488-nm elastic scattering. In (a) the short-wavelength side of the tyrosine line shape is affected by the transmission of the long-pass optical filter.

Fig. 4
Fig. 4

Three conditional logic cases set according to the two PMT’s voltage thresholds: (a) When only the elastic-scattering signal is used, both green- and red-fluorescence spectra are recorded; (b) when only the red-fluorescence signal is used, the fluorescence spectra from the red-dyed spheres alone are recorded; and (c) when the elastic-scattering NAND red-fluorescence signals are used, only the fluorescence spectra from the green-dyed spheres are recorded.

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