Abstract

We present a technique for observing single fluorophore molecules in solution. A mode-locked laser beam is focused into the solution, thereby defining a two-photon excitation volume localized in three dimensions. Molecules diffusing into and out of this volume produce fluorescence bursts, which are detected with a high signal-to-background ratio. The theoretical foundations for the technique are laid out, including the attendant fluorescence rate distribution, and agree with experimental results obtained for Rhodamine B molecules in water.

© 1995 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Single-molecule detection using continuous wave excitation of two-photon fluorescence

Ximiao Hou and Wei Cheng
Opt. Lett. 36(16) 3185-3187 (2011)

Single-molecule detection with axial flow into a micrometer-sized capillary

David A. Ball, Guoqing Shen, and Lloyd M. Davis
Appl. Opt. 46(7) 1157-1164 (2007)

Single-molecule analysis of ultradilute solutions with guided streams of 1-µm water droplets

Chung-yi Kung, Michael D. Barnes, Noah Lermer, William B. Whitten, and J. Michael Ramsey
Appl. Opt. 38(9) 1481-1487 (1999)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription