Abstract

The concept for a new type of confocal microscope with a fiber-optic imaging bundle is presented, and experimental results are shown to demonstrate the principle. The primary advantage of the system is the flexbility of imaging samples that would otherwise be inaccessible to confocal microscopy.

© 1993 Optical Society of America

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References

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  1. T. Wilson, Confocal Microscopy (Academic, London, 1990).
  2. E. H. K. Stelzer, in The Handbook of Biological Confocal Microscopy, J. Pawley, ed. (Plenum, New York, 1989), pp. 93–103.
  3. M. Gu, C. J. R. Sheppard, X. Gan, J. Opt. Soc. Am. A 8, 1755 (1991).
    [CrossRef]
  4. T. Dabbs, M. Glass, Appl. Opt. 31, 3030 (1992).
    [CrossRef] [PubMed]
  5. T. Wilson, A. R. Carlini, Opt. Lett. 12, 227 (1987).
    [CrossRef] [PubMed]

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Other (2)

T. Wilson, Confocal Microscopy (Academic, London, 1990).

E. H. K. Stelzer, in The Handbook of Biological Confocal Microscopy, J. Pawley, ed. (Plenum, New York, 1989), pp. 93–103.

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Figures (3)

Fig. 1
Fig. 1

Confocal microscope equipped with a fiber-optic imaging bundle. The components shown within the dotted line are those of a standard confocal microscope.

Fig. 2
Fig. 2

Fluorescence images obtained with the system of Fig. 1. (a) In-focus image for the confocal mode, (b) in-focus image for the nonconfocal mode, (c) out-of-focus image for the confocal mode, (d) out-of-focus image for the nonconfocal mode.

Fig. 3
Fig. 3

On-axis intensity distribution as a plane mirror is scanned through focus (reflection mode) for the standard confocal microscope (▲) and the fiber-optic imaging-bundle confocal microscope (●).

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