Abstract

Simultaneous imaging of laser-induced fluorescence in three selected wavelength bands from hematoporphyrin-derivative-bearing tissue has been performed, permitting considerable contrast enhancement for cancer-tumor localization.

© 1985 Optical Society of America

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References

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  1. T. J. Dougherty, in CRC Critical Reviews in Oncology/Hematology, S. Davis, ed. (CRC, Boca Raton, Fla., 1984).
  2. J. H. Kinsey, D. A. Cortese, Rev. Sci. Instrum. 51, 1403 (1980.
    [CrossRef] [PubMed]
  3. A. E. Profio, D. R. Doiron, E. G. King, Med. Phys. 6, 523 (1979);A. E. Profio, D. R. Doiron, O. J. Balchum, G. C. Huth, Med. Phys. 10, 35 (1983).
    [CrossRef] [PubMed]
  4. J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, Appl. Spectrosc. 38, 890 (1984).
    [CrossRef]
  5. J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).
  6. M. Aldén, H. Edner, S. Svanberg, Appl. Phys. B 29, 93 (1982).
    [CrossRef]
  7. S. Svanberg, J. Phys. Malaysia (to be published).
  8. J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).
  9. A. E. Profio, in Porphyrins in Tumor Phototherapy, A. Andreoni, R. Cubeddu, eds. (Plenum, New York, 1984).
  10. P. Herder, T. Olsson, E. Sjöblom, S. Svanberg, Lund Reports on Atomic Physics LRAP-9 (Lund University, Lund, Sweden, 1981).
  11. S. Montán, S. Svanberg, presented at the ICALEO '84 Symposium on Laser Diagnostics and Photochemistry, Boston, Mass., November 12–15 1984 (proceedings to be published by Laser Institute of America, Toledo, Ohio).

1984 (2)

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, Appl. Spectrosc. 38, 890 (1984).
[CrossRef]

1982 (1)

M. Aldén, H. Edner, S. Svanberg, Appl. Phys. B 29, 93 (1982).
[CrossRef]

1980 (1)

J. H. Kinsey, D. A. Cortese, Rev. Sci. Instrum. 51, 1403 (1980.
[CrossRef] [PubMed]

1979 (1)

A. E. Profio, D. R. Doiron, E. G. King, Med. Phys. 6, 523 (1979);A. E. Profio, D. R. Doiron, O. J. Balchum, G. C. Huth, Med. Phys. 10, 35 (1983).
[CrossRef] [PubMed]

Aldén, M.

M. Aldén, H. Edner, S. Svanberg, Appl. Phys. B 29, 93 (1982).
[CrossRef]

Ankerst, J.

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, Appl. Spectrosc. 38, 890 (1984).
[CrossRef]

J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).

Cortese, D. A.

J. H. Kinsey, D. A. Cortese, Rev. Sci. Instrum. 51, 1403 (1980.
[CrossRef] [PubMed]

Doiron, D. R.

A. E. Profio, D. R. Doiron, E. G. King, Med. Phys. 6, 523 (1979);A. E. Profio, D. R. Doiron, O. J. Balchum, G. C. Huth, Med. Phys. 10, 35 (1983).
[CrossRef] [PubMed]

Dougherty, T. J.

T. J. Dougherty, in CRC Critical Reviews in Oncology/Hematology, S. Davis, ed. (CRC, Boca Raton, Fla., 1984).

Edner, H.

M. Aldén, H. Edner, S. Svanberg, Appl. Phys. B 29, 93 (1982).
[CrossRef]

Herder, P.

P. Herder, T. Olsson, E. Sjöblom, S. Svanberg, Lund Reports on Atomic Physics LRAP-9 (Lund University, Lund, Sweden, 1981).

King, E. G.

A. E. Profio, D. R. Doiron, E. G. King, Med. Phys. 6, 523 (1979);A. E. Profio, D. R. Doiron, O. J. Balchum, G. C. Huth, Med. Phys. 10, 35 (1983).
[CrossRef] [PubMed]

Kinsey, J. H.

J. H. Kinsey, D. A. Cortese, Rev. Sci. Instrum. 51, 1403 (1980.
[CrossRef] [PubMed]

Montán, S.

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, Appl. Spectrosc. 38, 890 (1984).
[CrossRef]

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).

J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).

S. Montán, S. Svanberg, presented at the ICALEO '84 Symposium on Laser Diagnostics and Photochemistry, Boston, Mass., November 12–15 1984 (proceedings to be published by Laser Institute of America, Toledo, Ohio).

Olsson, T.

P. Herder, T. Olsson, E. Sjöblom, S. Svanberg, Lund Reports on Atomic Physics LRAP-9 (Lund University, Lund, Sweden, 1981).

Profio, A. E.

A. E. Profio, D. R. Doiron, E. G. King, Med. Phys. 6, 523 (1979);A. E. Profio, D. R. Doiron, O. J. Balchum, G. C. Huth, Med. Phys. 10, 35 (1983).
[CrossRef] [PubMed]

A. E. Profio, in Porphyrins in Tumor Phototherapy, A. Andreoni, R. Cubeddu, eds. (Plenum, New York, 1984).

Sjöblom, E.

P. Herder, T. Olsson, E. Sjöblom, S. Svanberg, Lund Reports on Atomic Physics LRAP-9 (Lund University, Lund, Sweden, 1981).

Sjöholm, E.

J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).

Svanberg, K.

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, Appl. Spectrosc. 38, 890 (1984).
[CrossRef]

J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).

Svanberg, S.

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, Appl. Spectrosc. 38, 890 (1984).
[CrossRef]

M. Aldén, H. Edner, S. Svanberg, Appl. Phys. B 29, 93 (1982).
[CrossRef]

S. Svanberg, J. Phys. Malaysia (to be published).

J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).

P. Herder, T. Olsson, E. Sjöblom, S. Svanberg, Lund Reports on Atomic Physics LRAP-9 (Lund University, Lund, Sweden, 1981).

S. Montán, S. Svanberg, presented at the ICALEO '84 Symposium on Laser Diagnostics and Photochemistry, Boston, Mass., November 12–15 1984 (proceedings to be published by Laser Institute of America, Toledo, Ohio).

Appl. Phys. B (1)

M. Aldén, H. Edner, S. Svanberg, Appl. Phys. B 29, 93 (1982).
[CrossRef]

Appl. Spectrosc. (1)

J. Opt. Soc. Am. B (1)

J. Ankerst, S. Montán, K. Svanberg, S. Svanberg, J. Opt. Soc. Am. B 1, 558 (1984).

Med. Phys. (1)

A. E. Profio, D. R. Doiron, E. G. King, Med. Phys. 6, 523 (1979);A. E. Profio, D. R. Doiron, O. J. Balchum, G. C. Huth, Med. Phys. 10, 35 (1983).
[CrossRef] [PubMed]

Rev. Sci. Instrum. (1)

J. H. Kinsey, D. A. Cortese, Rev. Sci. Instrum. 51, 1403 (1980.
[CrossRef] [PubMed]

Other (6)

T. J. Dougherty, in CRC Critical Reviews in Oncology/Hematology, S. Davis, ed. (CRC, Boca Raton, Fla., 1984).

S. Svanberg, J. Phys. Malaysia (to be published).

J. Ankerst, S. Montán, E. Sjöholm, K. Svanberg, S. Svanberg, presented at the ICALEO '84 Symposium on Lasers in Medicine and Biology, Boston, Mass., November 12–15,1984(proceedings to be published by Laser Institute of America, Toledo, Ohio).

A. E. Profio, in Porphyrins in Tumor Phototherapy, A. Andreoni, R. Cubeddu, eds. (Plenum, New York, 1984).

P. Herder, T. Olsson, E. Sjöblom, S. Svanberg, Lund Reports on Atomic Physics LRAP-9 (Lund University, Lund, Sweden, 1981).

S. Montán, S. Svanberg, presented at the ICALEO '84 Symposium on Laser Diagnostics and Photochemistry, Boston, Mass., November 12–15 1984 (proceedings to be published by Laser Institute of America, Toledo, Ohio).

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Figures (4)

Fig. 1
Fig. 1

Experimental arrangement for spatially resolved multicolor LIF measurements.

Fig. 2
Fig. 2

Illustration of spatially resolved LIF measurements across a tumor of a rat injected with 0.5 mg/kg of body weight of HPD two days earlier. The figure insert showing the tumor and the image line has the correct scale and pertains to all three columns. a, Fluorescence spectrum at the center of the tumor. b, Response functions in the different bands (recording of the fluorescence from a paper). c, Raw data from tumor recording. d, Corrected tumor data. e, Curve obtained (A) after subtraction of properly multiplied 600-nm curve (D) from 630-nm curve (A′). f, Data obtained in corresponding pointwise, spectrally resolved measurements.

Fig. 3
Fig. 3

Examples of contrast enhancement at tumor edges obtained by fitting D and A′ curves in the (distant) nontumor region with subsequent subtraction of the D curve. Rats were injected with 5 mg/kg of body weight of HPD one to three days before the investigation for data shown in the curves in the upper row and with 0.5 mg/kg for data in the lower row. Intensity scales differ; only contrast (and noise) is considered.

Fig. 4
Fig. 4

Recordings of fluorescence along a tissue line extending from muscle to blood-stained tumor; 5 mg/kg of body weight of HPD was injected one day before the investigation. a, Corrected fluorescence curves. b, The A curve obtained by subtraction of red curves. c, A/B ratio curve.

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