Abstract

We show that phase-sensitive detection of spectral hole refilling can yield information about self-phase modulation and two-photon absorption coefficients. We expect that, when applied to tissue microscopy, this technique will allow the study of endogenous molecular markers beneath the surface, even if those markers are nonfluorescent.

© 2005 Optical Society of America

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References

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    [CrossRef] [PubMed]

2003

W. S. Warren, W. Wagner, and T. Ye, Magn. Reson. Imaging 21, 1225 (2003).
[CrossRef]

2002

2000

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

1994

1990

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Berland, K. M.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Dong, C. Y.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Goswami, D.

Hagan, D. J.

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

Hillegas, C.

Lakowicz, J. R.

J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd ed. (Kluwer Academic/Plenum, New York, 1999).
[CrossRef]

Masters, B. R.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Miller, A. E.

A. E. Miller, “Two-photon absorption microscopy: selective detection of two-photon absorption and self-phase modulation with shaped ultrafast laser pulses,” bachelor’s thesis (Princeton University, Princeton, N.J., 2004).

Said, A. A.

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

Sheik-Bahae, M.

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

So, P. T. C.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

Strickland, D.

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Tian, P.

Tull, J. X.

van Stryland, E. W.

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

Wagner, W.

W. S. Warren, W. Wagner, and T. Ye, Magn. Reson. Imaging 21, 1225 (2003).
[CrossRef]

Warren, W. S.

Webb, W. W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Wei, T.-H.

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

Ye, T.

W. S. Warren, W. Wagner, and T. Ye, Magn. Reson. Imaging 21, 1225 (2003).
[CrossRef]

Annu. Rev. Biomed. Eng.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Rev. Biomed. Eng. 2, 399 (2000).
[CrossRef]

IEEE J. Quantum Electron.

M. Sheik-Bahae, A. A. Said, T.-H. Wei, D. J. Hagan, and E. W. van Stryland, IEEE J. Quantum Electron. 26, 760 (1990).
[CrossRef]

Magn. Reson. Imaging

W. S. Warren, W. Wagner, and T. Ye, Magn. Reson. Imaging 21, 1225 (2003).
[CrossRef]

Opt. Lett.

Science

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Other

J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd ed. (Kluwer Academic/Plenum, New York, 1999).
[CrossRef]

A. E. Miller, “Two-photon absorption microscopy: selective detection of two-photon absorption and self-phase modulation with shaped ultrafast laser pulses,” bachelor’s thesis (Princeton University, Princeton, N.J., 2004).

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Figures (3)

Fig. 1
Fig. 1

Pulse shape before (dashed curves) and after (solid curves) a TPA sample in (a) the frequency domain and (b) the time domain. (c) Square hole with a local oscillator (LO) component.

Fig. 2
Fig. 2

Experimental setup for the phase measurements: AOM, acousto-optic modulator.

Fig. 3
Fig. 3

Relative phase shift between a 30 - mM solution of R6G in MeOH and pure MeOH.

Equations (5)

Equations on this page are rendered with MathJax. Learn more.

E ( z , τ ) z = ( i η 2 α 2 2 ) E ( z , τ ) 2 E ( z , τ ) .
Δ E ̃ = z ( i η 2 α 2 2 ) E 2 E ˜ ,
E ̃ hom = E ̃ LO exp ( i ϕ ) + Δ E ̃ ,
E ̃ hom 2 ϕ = 2 z E ̃ LO E 2 E ˜ ( η 2 sin ϕ + α 2 2 cos ϕ ) .
tan ( Δ ϕ ) = α 2 ( 2 η 2 ) .

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