Abstract

It is demonstrated that high spatial frequency filtering of time domain fluorescence signals can allow efficient detection of intrinsic fluorescence lifetimes from turbid media and the rejection of diffuse excitation leakage. The basis of this approach is the separation of diffuse fluorescence signals into diffuse and fluorescent components with distinct spatiotemporal behavior.

© 2013 Optical Society of America

Full Article  |  PDF Article
Related Articles
Mathematical model for time-resolved and frequency-domain fluorescence spectroscopy in biological tissues

Michael S. Patterson and Brian W. Pogue
Appl. Opt. 33(10) 1963-1974 (1994)

Experimental verification of a theory for the time-resolved fluorescence spectroscopy of thick tissues

Albert E. Cerussi, John S. Maier, Sergio Fantini, Maria Angela Franceschini, William W. Mantulin, and Enrico Gratton
Appl. Opt. 36(1) 116-124 (1997)

Measurement of the fluorescence lifetime in scattering media by frequency-domain photon migration

Ralf H. Mayer, Jeffery S. Reynolds, and Eva M. Sevick-Muraca
Appl. Opt. 38(22) 4930-4938 (1999)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription