Abstract

In vivo high-resolution images are the most direct way to understand retinal function and diseases. Here we report the use of visible-light optical coherence tomography with volumetric registration and averaging to achieve cellular-level retinal structural imaging in a rat eye, covering the entire depth of the retina. Vitreous fibers, nerve fiber bundles, and vasculature were clearly revealed, as well as at least three laminar sublayers in the inner plexiform layer. We also successfully visualized ganglion cell somas in the ganglion cell layer, cells in the inner nuclear layer, and photoreceptors in the outer nuclear layer and ellipsoid zone. This technique provides, to the best of our knowledge, a new means to visualize the retina in vivo at a cellular resolution and may enable detection or discovery of cellular neuronal biomarkers to help better diagnose ocular disease.

© 2020 Optical Society of America

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Supplementary Material (5)

NameDescription
» Visualization 1       Visualization 1 B-scan changes in image quality when different numbers of volumes were averaged.
» Visualization 2       Visualization 2 En face image of nerve fiber layer changes in image quality when different numbers of volumes were averaged.
» Visualization 3       Visualization 3 En face image of ganglion cells changes in image quality when different numbers of volumes were averaged.
» Visualization 4       Visualization 4 B-scan fly through of the averaged structural volume.
» Visualization 5       Visualization 5 B-scan fly through of the merged angiographic volume.

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Figures (4)

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