Abstract

This Letter demonstrates a method to simultaneously measure the quantitative-phase signal (QPS) of the observed specimen and the refractive index of its surrounding medium (${{n}_{m}}$) in a time-resolved manner using a micro-structured coverslip. Such coverslips, easily integrated into perfused live-cell imaging chambers, allow to use various quantitative-phase imaging techniques to achieve this dual measurement. Since QPS is crucially dependent on ${{n}_{m}}$, the measurement of the latter paves the way for its manipulation in a controlled manner leading to a QPS contrast modulation for appealing applications, including visualizing the interior of cells.

© 2020 Optical Society of America

Phase contrast-based phase retrieval: a bridge between qualitative phase contrast and quantitative phase imaging by phase retrieval algorithms

Nathaniel Hai and Joseph Rosen
Opt. Lett. 45(20) 5812-5815 (2020)

Cross-sectional refractive-index variations in fiber Bragg gratings measured by quantitative phase imaging

Grayson M. Noah, Yijun Bao, and Thomas K. Gaylord
Opt. Lett. 45(1) 53-56 (2020)

Phase-shifting by means of an electronically tunable lens: quantitative phase imaging of biological specimens with digital holographic microscopy

Carlos Trujillo, Ana Doblas, Genaro Saavedra, Manuel Martínez-Corral, and Jorge García-Sucerquia
Opt. Lett. 41(7) 1416-1419 (2016)

Dual-wavelength surface plasmon resonance holographic microscopy for simultaneous measurements of cell-substrate distance and cytoplasm refractive index

Siqing Dai, Jingyu Mi, Jiazhen Dou, Tongyao Yu, Mengmeng Zhang, Jianglei Di, Jiwei Zhang, and Jianlin Zhao
Opt. Lett. 47(9) 2306-2309 (2022)

Quantitative phase imaging based on wavefront correction of a digital micromirror device

Jing Hu, Xiwei Xie, and Yibing Shen
Opt. Lett. 45(18) 5036-5039 (2020)